概述

  • 产品名称
    Anti-UBE1C抗体[MILR42872]
    参阅全部 UBE1C 一抗
  • 描述
    兔单克隆抗体[MILR42872] to UBE1C
  • 经测试应用
    适用于: WBmore details
    不适用于: Flow Cyt,ICC/IF,IHC-P or IP
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    A synthetic peptide corresponding to residues in human UBE1C

  • 阳性对照
    • Lysates of: fetal lung, 293T, HeLa, RAW 264.7, HepG2, K562
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

性能

应用

Our Abpromise guarantee covers the use of ab124728 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/10000 - 1/50000. Predicted molecular weight: 49 kDa.
  • 应用说明
    Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
  • 靶标

    • 功能
      Catalytic subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Down-regulates steroid receptor activity. Necessary for cell cycle progression.
    • 组织特异性
      Ubiquitously expressed.
    • 通路
      Protein modification; protein neddylation.
    • 序列相似性
      Belongs to the ubiquitin-activating E1 family. UBA3 subfamily.
    • Information by UniProt
    • 数据库链接
    • 别名
      • DKFZp566J164 antibody
      • EC 6.3.2. antibody
      • hUba3 antibody
      • MGC22384 antibody
      • NEDD8 activating enzyme E1 catalytic subunit antibody
      • NEDD8 activating enzyme E1C antibody
      • Nedd8 activating enzyme hUba3 antibody
      • NEDD8-activating enzyme E1 catalytic subunit antibody
      • NEDD8-activating enzyme E1C antibody
      • uba3 antibody
      • UBA3 ubiquitin activating enzyme E1 homolog antibody
      • UBA3_HUMAN antibody
      • UBE1C antibody
      • Ubiquitin activating enzyme 3 antibody
      • Ubiquitin activating enzyme E1C antibody
      • Ubiquitin-activating enzyme 3 antibody
      • Ubiquitin-activating enzyme E1C antibody
      • Ubiquitin-like modifier-activating enzyme 3 antibody
      see all

    Anti-UBE1C antibody [MILR42872] 图像

    • All lanes : Anti-UBE1C antibody [MILR42872] (ab124728)

      Lane 1 : Fetal lung cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : RAW 264.7 cell lysate
      Lane 5 : HepG2 cell lysate
      Lane 6 : K562 cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 49 kDa

    实验方案

    Anti-UBE1C antibody [MILR42872] (ab124728)参考文献

    This product has been referenced in:
    • Coleman KE  et al. SENP8 limits aberrant neddylation of NEDD8 pathway components to promote cullin-RING ubiquitin ligase function. Elife 6:N/A (2017). Read more (PubMed: 28475037) »
    • Xu J  et al. The neddylation-cullin 2-RBX1 E3 ligase axis targets tumor suppressor RhoB for degradation in liver cancer. Mol Cell Proteomics 14:499-509 (2015). Read more (PubMed: 25540389) »

    See all 2 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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