概述

  • 产品名称Anti-UBE1C抗体
    参阅全部 UBE1C 一抗
  • 描述
    兔多克隆抗体to UBE1C
  • 经测试应用适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Cow, Cat, Dog, Pig, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to a region within the N terminal amino acids 36-85 (GRWNHVKKFL ERSGPFTHPD FEPSTESLQF LLDTCKVLVI GAGGLGCELL) of Human UBE1C (NP_003959).

  • 阳性对照
    • Human fetal pancreas lysate

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab87791 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 2 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 52 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能Catalytic subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Down-regulates steroid receptor activity. Necessary for cell cycle progression.
  • 组织特异性Ubiquitously expressed.
  • 通路Protein modification; protein neddylation.
  • 序列相似性Belongs to the ubiquitin-activating E1 family. UBA3 subfamily.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DKFZp566J164 antibody
    • EC 6.3.2. antibody
    • hUba3 antibody
    • MGC22384 antibody
    • NEDD8 activating enzyme E1 catalytic subunit antibody
    • NEDD8 activating enzyme E1C antibody
    • Nedd8 activating enzyme hUba3 antibody
    • NEDD8-activating enzyme E1 catalytic subunit antibody
    • NEDD8-activating enzyme E1C antibody
    • uba3 antibody
    • UBA3 ubiquitin activating enzyme E1 homolog antibody
    • UBA3_HUMAN antibody
    • UBE1C antibody
    • Ubiquitin activating enzyme 3 antibody
    • Ubiquitin activating enzyme E1C antibody
    • Ubiquitin-activating enzyme 3 antibody
    • Ubiquitin-activating enzyme E1C antibody
    • Ubiquitin-like modifier-activating enzyme 3 antibody
    see all

Anti-UBE1C antibody 图像

  • Anti-UBE1C antibody (ab87791) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal pancreas lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 52 kDa
    Observed band size : ~45 kDa (why is the actual band size different from the predicted?)
  • Immunofluorescence analysis of Human brain stem cells staining UBE1C with ab87791 at 2 µg/ml.

  • All lanes : Anti-UBE1C antibody (ab87791) at 2 µg/ml

    Lane 1 : Human NT-2 cells at 60 µg
    Lane 2 : Mouse brain extracts at 80 µg


    Predicted band size : 52 kDa

Anti-UBE1C antibody (ab87791)参考文献

ab87791 has not yet been referenced specifically in any publications.

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