概述

  • 产品名称Anti-UBC6e抗体
    参阅全部 UBC6e 一抗
  • 描述
    兔多克隆抗体to UBC6e
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Chicken, Guinea pig, Cat, Pig, Zebrafish
  • 免疫原

    A region within synthetic peptide: METRYNLKSP AVKRLMKEAA ELKDPTDHYH AQPLEDNLFE WHFTVRGPPD, corresponding to N terminal amino acids 1-50 of Human UBC6e

  • 阳性对照
    • Jurkat cell lysate, Human lung tissue, Human kidney tissue.

性能

应用

Our Abpromise guarantee covers the use of ab59014 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 2.5 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 35 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.

靶标

  • 功能Catalyzes the covalent attachment of ubiquitin to other proteins. Seems to function in the selective degradation of misfolded membrane proteins from the endoplasmic reticulum.
  • 通路Protein modification; protein ubiquitination.
  • 序列相似性Belongs to the ubiquitin-conjugating enzyme family.
  • 细胞定位Endoplasmic reticulum membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CGI-76 antibody
    • HSPC153 antibody
    • HSPC205 antibody
    • HSU93243 antibody
    • HSUBC6e antibody
    • MGC12555 antibody
    • NCUBE-1 antibody
    • NCUBE1 antibody
    • Non-canonical ubiquitin-conjugating enzyme 1 antibody
    • UB2J1_HUMAN antibody
    • UBC6 antibody
    • UBC6E antibody
    • Ubc6p antibody
    • Ube2j1 antibody
    • Ubiquitin conjugating enzyme E2, J1 (UBC6 homolog, yeast) antibody
    • Ubiquitin conjugating enzyme E2, J1, U antibody
    • Ubiquitin-conjugating enzyme E2 J1 antibody
    • Yeast ubiquitin-conjugating enzyme UBC6 homolog E antibody
    see all

Anti-UBC6e antibody 图像

  • All lanes : Anti-UBC6e antibody (ab59014) at 2.5 µg/ml

    Lane 1 : Molecular weight markers
    Lane 2 : Jurkat cell lysate at 10 µg/ml

    Secondary
    Lane 2 : HRP conjugated anti-Rabbit IgG at 1/50000 dilution


    Predicted band size : 35 kDa
    Observed band size : 35 kDa
    Diluted in 5% skim milk / PBS buffer; gel concentration 12%
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung tissue labelling UBC6e with ab59014 at 4-8µg/ml. Arrows indicate positively labelled alveolar cells. Magnification: 400X.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling UBC6e with ab59014 at 4-8µg/ml. Arrows indicate positively labelled epithelial cells of renal tubule. Magnification: 400X.

Anti-UBC6e antibody (ab59014)参考文献

ab59014 has not yet been referenced specifically in any publications.

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