概述

  • 产品名称Anti-UBASH3A抗体
    参阅全部 UBASH3A 一抗
  • 描述
    山羊多克隆抗体to UBASH3A
  • 特异性ab115033 is expected to recognize both reported isoforms (NP_061834.1; NP_001001895.1).
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Horse
  • 免疫原

    Synthetic peptide:

    C-DSGIRISSVFASP

    , corresponding to internal sequence amino acids 470-482 of Human UBASH3A (NP_061834.1).

  • 阳性对照
    • Human spleen lysate

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 99% Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明ab115033 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab115033 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 74 kDa).
IHC-P Use a concentration of 3 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能Interferes with CBL-mediated down-regulation and degradation of receptor-type tyrosine kinases. Promotes accumulation of activated target receptors, such as T-cell receptors, EGFR and PDGFRB, on the cell surface. Exhibits negligigle protein tyrosine phosphatase activity at neutral pH. May act as a dominant-negative regulator of UBASH3B-dependent dephosphorylation. May inhibit dynamin-dependent endocytic pathways by functionally sequestering dynamin via its SH3 domain.
  • 组织特异性Highest expression of UBASH3A in tissues belonging to the immune system, including spleen, peripheral blood leukocytes, thymus and bone marrow.
  • 序列相似性Contains 1 SH3 domain.
    Contains 1 UBA domain.
  • 细胞定位Cytoplasm. Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Cbl interacting protein 4 antibody
    • Cbl-interacting protein 4 antibody
    • CLIP4 antibody
    • STS 2 antibody
    • STS-2 antibody
    • Suppressor of T cell receptor signaling 2 antibody
    • Suppressor of T-cell receptor signaling 2 antibody
    • T cell ubiquitin ligand antibody
    • T cell ubiquitin ligand protein antibody
    • T-cell ubiquitin ligand 1 antibody
    • TULA antibody
    • TULA-1 antibody
    • UBASH3A antibody
    • ubiquitin associated and SH3 domain containing, A antibody
    • Ubiquitin-associated and SH3 domain-containing protein A antibody
    • UBS3A_HUMAN antibody
    see all

Anti-UBASH3A antibody 图像

  • Anti-UBASH3A antibody (ab115033) at 1 µg/ml + Human spleen lysate (in RIPA buffer) at 35 µg
    Developed using the ECL technique

    Predicted band size : 74 kDa
    Observed band size : 70 kDa (why is the actual band size different from the predicted?)
  • ab115033 (3.8µg/ml) staining of paraffin embedded Human Breast shows perinuclear staining in the epithelial cells of the lubules. Tissue treatment: Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Anti-UBASH3A antibody (ab115033)参考文献

ab115033 has not yet been referenced specifically in any publications.

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