概述

  • 产品名称Anti-UBA52抗体[EPR4546]
    参阅全部 UBA52 一抗
  • 描述
    兔单克隆抗体[EPR4546] to UBA52
  • 经测试应用适用于: WB, IP, IHC-P, ICC/IFmore details
    不适用于: Flow Cyt
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide from the N-terminus of Human UBA52.

  • 阳性对照
    • Fetal kidney, Human placenta, 293T and JAR cell lysates. Human brain tissue. SH-SY-5Y cells.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

性能

应用

Our Abpromise guarantee covers the use of ab109227 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Predicted molecular weight: 15 kDa.
IP 1/10 - 1/100.
IHC-P 1/500 - 1/1000. Perform antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/250 - 1/500.
  • 应用说明Is unsuitable for Flow Cyt.
  • 靶标

    • 相关性Ubiquitin is a highly conserved nuclear and cytoplasmic protein that has a major role in targeting cellular proteins for degradation by the 26S proteosome. It is also involved in the maintenance of chromatin structure, the regulation of gene expression, and the stress response. Ubiquitin is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin moiety fused to an unrelated protein. UBA52 is a fusion protein consisting of ubiquitin at the N terminus and ribosomal protein L40 at the C terminus, a C-terminal extension protein (CEP). Multiple processed pseudogenes derived from this gene are present in the genome.
    • 细胞定位Ubiquitin: Cytoplasm. Nucleus. 60S ribosomal protein L40: Cytoplasm
    • 数据库链接
    • 别名
      • 60S ribosomal protein L40 antibody
      • CEP52 antibody
      • HUBCEP52 antibody
      • L40 antibody
      • MGC126879 antibody
      • MGC57125 antibody
      • RPL40 antibody
      • UBA 52 antibody
      • Ubiquitin 52 amino acid fusion protein antibody
      • Ubiquitin 60S ribosomal protein L40 antibody
      • Ubiquitin A 52 residue ribosomal protein fusion product 1 antibody
      • Ubiquitin carboxyl extension protein 52 antibody
      • Ubiquitin CEP52 antibody
      see all

    Anti-UBA52 antibody [EPR4546] 图像

    • All lanes : Anti-UBA52 antibody [EPR4546] (ab109227) at 1/1000 dilution

      Lane 1 : fetal kidney lysates
      Lane 2 : Human placenta lysates
      Lane 3 : 293T cell lysates
      Lane 4 : JAR cell lysates

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 15 kDa
      Observed band size : 10 kDa (why is the actual band size different from the predicted?)
    • Immunohistochemical analysis of paraffin-embedded Human brain tissue using 1/500 ab109227.
    • Immunofluorescent staining of SH-SY-5Y cells using ab109227 at 1/250

    Anti-UBA52 antibody [EPR4546] (ab109227)参考文献

    This product has been referenced in:
    • Francis C  et al. Divergence of RNA localization between rat and mouse neurons reveals the potential for rapid brain evolution. BMC Genomics 15:883 (2014). ICC ; Mouse, Rat . Read more (PubMed: 25301173) »

    See 1 Publication for this product

    Product Wall

    Thank you for your enquiry.

    I am sorry to confirm that the cross-reactivity of the ab109227 UBA52 antibody to other Ubiquitin proteins have not been verified by testing. However, based on sequence analysis, the immunogen does not share seque...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"