Anti-UAP1抗体(ab95949)
Key features and details
- Rabbit polyclonal to UAP1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-UAP1抗体
参阅全部 UAP1 一抗 -
描述
兔多克隆抗体to UAP1 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Cow, Zebrafish -
免疫原
Recombinant protein fragment containing a sequence corresponding to a region within amino acids 53-473 of UAP1 isoform AGX1 (Human)
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阳性对照
- H1299 lysate, A549 cells (IF), and ES-2 xenograft (IHC)
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab95949于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/500 - 1/3000. Predicted molecular weight: 57 kDa.
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IHC-P |
1/100 - 1/500.
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ICC/IF |
1/100 - 1/200.
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说明 |
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WB
1/500 - 1/3000. Predicted molecular weight: 57 kDa. |
IHC-P
1/100 - 1/500. |
ICC/IF
1/100 - 1/200. |
靶标
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功能
Converts UDP and GlcNAc-1-P into UDP-GlcNAc, and UDP and GalNAc-1-P into UDP-GalNAc. Isoform AGX1 has 2 to 3 times higher activity towards GalNAc-1-P, while isoform AGX2 has 8 times more activity towards GlcNAc-1-P. -
组织特异性
Widely expressed. Isoform AGX1 is more abundant in testis than isoform AGX2, while isoform AGX2 is more abundant than isoform AGX1 in somatic tissue. Expressed at low level in placenta, muscle and liver. -
通路
Nucleotide-sugar biosynthesis; UDP-N-acetyl-alpha-D-glucosamine biosynthesis; UDP-N-acetyl-alpha-D-glucosamine from N-acetyl-alpha-D-glucosamine 1-phosphate: step 1/1. -
序列相似性
Belongs to the UDPGP type 1 family. -
细胞定位
Cytoplasm. In spermatozoa, localized to the principal piece of the tail, the neck region of the head and to a lesser extent, the midpiece of the tail. - Information by UniProt
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数据库链接
- Entrez Gene: 535325 Cow
- Entrez Gene: 6675 Human
- Entrez Gene: 107652 Mouse
- Omim: 602862 Human
- SwissProt: Q16222 Human
- SwissProt: Q91YN5 Mouse
- Unigene: 492859 Human
- Unigene: 27969 Mouse
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别名
- AGX 1 antibody
- AGX antibody
- AGX-1 antibody
see all
图片
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Anti-UAP1 antibody (ab95949) at 1/3000 dilution + H1299 whole cell lysate at 30 µg
Predicted band size: 57 kDa
gel concentration: 7.5% -
Immunofluorescence analysis of paraformaldehyde fixed A549 cells using ab95949: (1) at 1/200 dilution, (2) merged with DNA probe.
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Immunohistochemical analysis of paraffin embedded ES 2 xenograft using ab95949 at 1/500 dilution.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab95949 被引用在 2 文献中.
- Feinberg D et al. Inhibition of O-GlcNAcylation Decreases the Cytotoxic Function of Natural Killer Cells. Front Immunol 13:841299 (2022). PubMed: 35479087
- Mason B et al. Fbxl17 is rearranged in breast cancer and loss of its activity leads to increased global O-GlcNAcylation. Cell Mol Life Sci 77:2605-2620 (2020). PubMed: 31560077