Application
Western blot
Sample
Mouse Tissue lysate - whole (Lungs)
Gel Running Conditions
Non-reduced Denaturing (8%)
Loading amount
40 µg
Specification
Lungs
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3%
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBST
Dilution
1/1000
Secondary antibody
Dilution
1/7500
Name
Non-Abcam antibody was used: Donkey anti rabbit
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
20 second(s)
Bands
Specific: 70 kDa Non-specific: 28 kDa
Additional data
Additional Notes
Works really great with octa-beta-D-Glucopyranoside as constituent for lysis buffer. Wild type mouse homogenate and Lung epithelial cells (b2b, human)
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提交于 Jul 12 2011