U937核extract裂解液(TPA stimulated) (ab14910)

概述

  • 产品名称U937核extract裂解液(TPA stimulated)
    参阅全部 U937 细胞裂解液
  • 描述
    U937核extract裂解液
  • 常规说明Cells were cultured in medium supplemented with 5 nM TPA (phorbol, 12-myristate, 13 acetate) for 72 hours at 37°C immediately prior to harvesting. Extracts have been quality control tested by Western blot and the Electrophoretic Mobility Shift Assay (EMSA). To ensure stability, we recommend aliquoting the extracts into single-use fractions and then storing them at -80ºC.

性能

  • 形式Liquid
  • 存放说明Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 20% Glycerol, 20mM HEPES, 100mM Potassium Chloride, 1mM Magnesium Chloride, 0.5mM PMSF, 0.5mM DTT. pH 7.9
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  • 研究领域
  • 背景The U937 cell line is a human cell line established from a diffuse histiocytic lymphoma and displaying many monocytic characteristics. It serves as an in vitro model for monocyte/macrophage differentiation, antitumour testing, tumorigenicity. U937 cells dominantly expressed the 44-46 kDa paxillin forms and IFN-beta and IFN-gamma stimulation lead to the accumulation of 66-70 kDa paxillin forms.

U937 nuclear extract lysate (TPA stimulated) (ab14910)参考文献

ab14910 has not yet been referenced specifically in any publications.

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