U937核extract裂解液(IFN alpha treated) (ab14907)

概述

  • 产品名称U937核extract裂解液(IFN alpha treated)
    参阅全部 U937 细胞裂解液
  • 描述
    U937核extract裂解液
  • 常规说明Cells were cultured in medium supplemented with 5000 U/mL interferon alpha for one hour at 37°C immediately prior to harvesting. Extracts have been quality control tested by Western blot and the Electrophoretic Mobility Shift Assay (EMSA). To ensure stability, we recommend aliquoting the extracts into single-use fractions and then storing them at -80ºC.

性能

  • 形式Liquid
  • 存放说明Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 20% Glycerol, 20mM HEPES, 100mM Potassium Chloride, 1mM Magnesium Chloride, 0.5mM PMSF, 0.5mM DTT. pH 7.9
  • Concentration information loading...
  • 研究领域
  • 背景The U937 cell line is a human cell line established from a diffuse histiocytic lymphoma and displaying many monocytic characteristics. It serves as an in vitro model for monocyte/macrophage differentiation, antitumour testing, tumorigenicity. U937 cells dominantly expressed the 44-46 kDa paxillin forms and IFN-beta and IFN-gamma stimulation lead to the accumulation of 66-70 kDa paxillin forms.

U937 nuclear extract lysate (IFN alpha treated) (ab14907)参考文献

ab14907 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your phone call the other day. I have heard back from the production lab regarding the IFN alpha treatment of the cell prior to lysis, and I received the following response: "The treatment with IFN alpha has no effect on the stabili...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"