概述

  • 产品名称Anti-U1SNRNPBP抗体
  • 描述
    兔多克隆抗体to U1SNRNPBP
  • 经测试应用适用于: WB, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Cow
  • 免疫原

    A region within synthetic peptide: VWRAMLARYV PNKGVIGDPL LTLFVARLNL QTKEDKLKEV FSRYGDIRRL, corresponding to N terminal amino acids 36-85 of Human U1SNRNPBP

  • 阳性对照
    • Jurkat cell lysate, Human kidney

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度Protein A purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab51035 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 2.5 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 29 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA 1/1.5625e+006.

Titre using peptide based assay 1/1562500.

IHC-P Use a concentration of 4 - 8 µg/ml.

靶标

  • 相关性U1SNRNPBP is a homolog of U1-snRNP binding protein. Its N-terminal contains an RNA recognition motif. The C-terminal is rich in Arg/Asp and Arg/Glu dipeptides, commonly found in splicing factors. Exists in at least two isoforms produced by alternative splicing that differ in the 5' sequence regions.
  • 细胞定位Nuclear
  • 数据库链接
  • 别名
    • HM 1 antibody
    • U1 snRNP binding protein homolog antibody
    • U11/U12 snRNP 35K antibody
    • U11/U12 snRNP 35K, isoform b antibody
    • U11/U12 snRNP antibody
    see all

Anti-U1SNRNPBP antibody 图像

  • Anti-U1SNRNPBP antibody (ab51035) at 2.5 µg/ml + Jurkat cell lysate at 10 µg/ml with skim milk/ PBS at 5 %

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 29 kDa
    Observed band size : 35 kDa (why is the actual band size different from the predicted?)
  • ab51035 at 4.0 µg/ml staining epithelial cells of renal tubules from human kidney; paraffin embedded; magnification 400x.

Anti-U1SNRNPBP antibody (ab51035)参考文献

This product has been referenced in:
  • Shen H  et al. The U2AF35-related protein Urp contacts the 3' splice site to promote U12-type intron splicing and the second step of U2-type intron splicing. Genes Dev 24:2389-94 (2010). WB ; Human . Read more (PubMed: 21041408) »

See 1 Publication for this product

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