概述

  • 产品名称Anti-U1A抗体
    参阅全部 U1A 一抗
  • 描述
    兔多克隆抗体to U1A
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Human, Drosophila melanogaster
    预测可用于: Cow
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Human U1A.

    (Peptide available as ab41553.)

  • 阳性对照
    • This antibody gave a positive signal in the following whole cell lysates: HeLa (Human epithelial carcinoma cell line) Jurkat (Human T cell lymphoblast-like cell line) A431 (Human epithelial carcinoma cell line) HEK293 (Human embryonic kidney cell line) MCF7 (Human breast adenocarcinoma cell line) SHSY-5Y (Human neuroblastoma cell line)

性能

  • 形式Liquid
  • 存放说明Shipped at 4��C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab40689 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 31 kDa).
ICC/IF Use a concentration of 5 µg/ml.

靶标

  • 功能Binds stem loop II of U1 snRNA. It is the first snRNP to interact with pre-mRNA. This interaction is required for the subsequent binding of U2 snRNP and the U4/U6/U5 tri-snRNP. In a snRNP-free form (SF-A) may be involved in coupled pre-mRNA splicing and polyadenylation process.
  • 序列相似性Belongs to the RRM U1 A/B'' family.
    Contains 2 RRM (RNA recognition motif) domains.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
    see all
  • 别名
    • fc19d01 antibody
    • Mud1 antibody
    • small nuclear ribonucleoprotein polypeptide A antibody
    • snRNP A antibody
    • snRNP protein A antibody
    • SNRPA antibody
    • SNRPA_HUMAN antibody
    • U1 small nuclear ribonucleoprotein A antibody
    • U1 small nuclear RNP specific A antibody
    • U1 snRNP A antibody
    • U1 snRNP specific protein A antibody
    • U1-A antibody
    • U1A antibody
    • wu:fc19d01 antibody
    • zgc:77810 antibody
    see all

Anti-U1A antibody 图像

  • All lanes : Anti-U1A antibody (ab40689) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 5 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 6 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 31 kDa
    Observed band size : 35 kDa (why is the actual band size different from the predicted?)
  • ICC/IF image of ab40689 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab40689, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
  • Anti-U1A antibody (ab40689) at 1/200 dilution + NSC34 cell nuclear extract at 20 µg

    Secondary
    IRDye® 800CW Donkey anti-Rabbit IgG at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 31 kDa
    Observed band size : 31 kDa


    Exposure time : 1 minute

    This image is courtesy of an Anonymous Abreview.

    See Abreview

Anti-U1A antibody (ab40689)参考文献

This product has been referenced in:
  • Brody Y  et al. The in vivo kinetics of RNA polymerase II elongation during co-transcriptional splicing. PLoS Biol 9:e1000573 (2011). ICC/IF ; Human . Read more (PubMed: 21264352) »
  • Bandhakavi S  et al. Quantitative nuclear proteomics identifies mTOR regulation of DNA damage response. Mol Cell Proteomics 9:403-14 (2010). WB ; Human . Read more (PubMed: 19955088) »

See all 2 Publications for this product

Product Wall

Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - nuclear (NSC34 cells)
Loading amount 20 µg
Specification NSC34 cells
Gel Running Conditions Reduced Denaturing (4-12% Nupage gel)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

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Verified customer

提交于 Dec 07 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Fruit fly (Drosophila melanogaster) Cell lysate - nuclear (OregonR embryo nuclear extract and S2 cell whole c)
Loading amount 40 µg
Specification OregonR embryo nuclear extract and S2 cell whole c
Gel Running Conditions Reduced Denaturing (15%)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Jul 08 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"