概述

  • 产品名称Anti-TZFP抗体
    参阅全部 TZFP 一抗
  • 描述
    小鼠多克隆抗体to TZFP
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant full length human TZFP with proprietary tag, amino acids 1-302 (AAH17700.1)

  • 阳性对照
    • Human colon tissue lysate, TZFP transfected 293T cell lysate, HeLa cells.

性能

应用

Our Abpromise guarantee covers the use of ab88594 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 53 kDa.
ICC/IF Use a concentration of 10 µg/ml.

靶标

  • 功能DNA-binding protein that binds to the to a 5'-TGTACAGTGT-3' core sequence. May function as a transcriptional transactivator and transcriptional repressor. Probably exerts its repressor effect by preventing GATA3 from binding to DNA. May play a role in regulating the differentiation and activation of helper T cells.
  • 组织特异性Predominantly expressed in testis. Some isoforms are ubiquitously expressed.
  • 序列相似性Belongs to the krueppel C2H2-type zinc-finger protein family.
    Contains 1 BTB (POZ) domain.
    Contains 3 C2H2-type zinc fingers.
  • 结构域The C-terminal zinc finger domain functions as a transcriptional transactivator.
    The BTB (POZ) domain possesses repressor activity.
  • 细胞定位Nucleus. Located in nuclear speckles.
  • Information by UniProt
  • 数据库链接
  • 别名
    • FANCC interacting protein antibody
    • FANCC-interacting protein antibody
    • Fanconi anemia zinc finger protein antibody
    • FAXF antibody
    • FAZF antibody
    • OTTHUMP00000045790 antibody
    • repressor of GATA antibody
    • Rog antibody
    • Testis zinc finger protein antibody
    • ZBT32_HUMAN antibody
    • Zbtb32 antibody
    • ZBTB32 protein antibody
    • Zinc finger and BTB domain containing 32 antibody
    • Zinc finger and BTB domain-containing protein 32 antibody
    • Zinc finger protein 538 antibody
    • ZNF538 antibody
    see all

Anti-TZFP antibody 图像

  • Anti-TZFP antibody (ab88594) at 5 µg/ml + Human colon tissue lysate at 50 µg

    Predicted band size : 53 kDa
    Observed band size : 53 kDa
  • All lanes : Anti-TZFP antibody (ab88594) at 5 µg/ml

    Lane 1 : TZFP transfected 293t cell lysate
    Lane 2 : Non-transfected lysate

    Lysates/proteins at 25 µg/ml per lane.


    Predicted band size : 53 kDa
    Observed band size : 33 kDa (why is the actual band size different from the predicted?)
  • ab88594 at 10µg/ml staining TZFP in HeLa cells by Immunofluorescence.

Anti-TZFP antibody (ab88594)参考文献

ab88594 has not yet been referenced specifically in any publications.

Product Wall

Application Immunoprecipitation
Immuno-precipitation step Protein G
Sample Human Cell lysate - whole cell (transfected 293T cells)
Specification transfected 293T cells
Total protein in input 2e+006 cells
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提交于 Feb 20 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"