Anti-Tyrosyl tRNA synthetase抗体[YAR5H08] (ab50961)


  • 产品名称Anti-Tyrosyl tRNA synthetase抗体[YAR5H08]
    参阅全部 Tyrosyl tRNA synthetase 一抗
  • 描述
    小鼠单克隆抗体[YAR5H08] to Tyrosyl tRNA synthetase
  • 经测试应用适用于: WB, IP, ICC, Dot blotmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Recombinant human YARS fragment

  • 阳性对照
    • HeLa cells and cell lysate and NIH3T3 cell lysate.


  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • 存储溶液Preservative: 0.05% Sodium Azide
    Constituents: 1% BSA, PBS, 3mM Potassium chloride, 140mM Sodium chloride, 1.5mM KH2PO4, 8.0mM Na2HPO4, pH 7.4
  • Concentration information loading...
  • 纯度Protein G purified
  • 纯化说明ab50961 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
  • 克隆单克隆
  • 克隆编号YAR5H08
  • 同种型IgG1
  • 研究领域


Our Abpromise guarantee covers the use of ab50961 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/100. Detects a band of approximately 58 kDa (predicted molecular weight: 58 kDa).
IP Use at 20 µg/mg of lysate.
ICC 1/10.
Dot blot Use at an assay dependent dilution.


  • 功能Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr).
  • 疾病相关Defects in YARS are the cause of Charcot-Marie-Tooth disease dominant intermediate type C (CMTDIC) [MIM:608323]. CMTDIC is a form of Charcot-Marie-Tooth disease characterized by clinical and pathologic features intermediate between demyelinating and axonal peripheral neuropathies, and motor median nerve conduction velocities ranging from 25 to 45 m/sec.
  • 序列相似性Belongs to the class-I aminoacyl-tRNA synthetase family.
    Contains 1 tRNA-binding domain.
  • 细胞定位Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CMTDIC antibody
    • SYYC_HUMAN antibody
    • Tyrosine tRNA ligase, cytoplasmic antibody
    • Tyrosine tRNA ligase 1, cytoplasmic antibody
    • Tyrosyl tRNA synthetase antibody
    • Tyrosyl--tRNA ligase antibody
    • Tyrosyl-tRNA synthetase, cytoplasmic antibody
    • TyrRS antibody
    • yars antibody
    • YRS antibody
    • YTS antibody
    see all

Anti-Tyrosyl tRNA synthetase antibody [YAR5H08] 图像

  • Anti-Tyrosyl tRNA synthetase antibody [YAR5H08] (ab50961) at 1/100 dilution + HeLa whole cell lysate at 50 µg

    anti-mouse IgG at 1/2500 dilution
    Developed using the ECL technique

    Predicted band size : 58 kDa
    Observed band size : 58 kDa
  • Immunoprecipititation analysis using 10ug ab50961 per 500ug RIPA HeLa cell lysate. Immunoblot using ab50961 at 1/100 dilution and TrueBlotTM anti-mouse IgG at 1/1000 as secondary antibody.
  • Anti-Tyrosyl tRNA synthetase antibody [YAR5H08] (ab50961) at 1/100 dilution + NIH3T3 whole cell lysate at 50 µg

    anti-mouse IgG at 1/2500 dilution
    Developed using the ECL technique

    Predicted band size : 58 kDa
    Observed band size : 58 kDa
  • ab50961 at 1/10 dilution staining human YARS in HeLa cells by Immunocytochemistry. AlexaFluor 488 Goat anti-mouse IgG at 1/200 as secondary.

Anti-Tyrosyl tRNA synthetase antibody [YAR5H08] (ab50961)参考文献

This product has been referenced in:
  • Park SJ  et al. Evaluation of Multi-tRNA Synthetase Complex by Multiple Reaction Monitoring Mass Spectrometry Coupled with Size Exclusion Chromatography. PLoS One 10:e0142253 (2015). WB ; Human . Read more (PubMed: 26544075) »

See 1 Publication for this product

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