Anti-Tyrosyl tRNA synthetase抗体[YAR5H08] (ab50961)

概述

  • 产品名称Anti-Tyrosyl tRNA synthetase抗体[YAR5H08]
    参阅全部 Tyrosyl tRNA synthetase 一抗
  • 描述
    小鼠单克隆抗体[YAR5H08] to Tyrosyl tRNA synthetase
  • 经测试应用适用于: WB, IP, ICC, Dot blotmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Recombinant human YARS fragment

  • 阳性对照
    • HeLa cells and cell lysate and NIH3T3 cell lysate.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • 存储溶液Preservative: 0.05% Sodium Azide
    Constituents: 1% BSA, PBS, 3mM Potassium chloride, 140mM Sodium chloride, 1.5mM KH2PO4, 8.0mM Na2HPO4, pH 7.4
  • Concentration information loading...
  • 纯度Protein G purified
  • 纯化说明ab50961 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
  • 克隆单克隆
  • 克隆编号YAR5H08
  • 同种型IgG1
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab50961 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/100. Detects a band of approximately 58 kDa (predicted molecular weight: 58 kDa).
IP Use at 20 µg/mg of lysate.
ICC 1/10.
Dot blot Use at an assay dependent dilution.

靶标

  • 功能Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr).
  • 疾病相关Defects in YARS are the cause of Charcot-Marie-Tooth disease dominant intermediate type C (CMTDIC) [MIM:608323]. CMTDIC is a form of Charcot-Marie-Tooth disease characterized by clinical and pathologic features intermediate between demyelinating and axonal peripheral neuropathies, and motor median nerve conduction velocities ranging from 25 to 45 m/sec.
  • 序列相似性Belongs to the class-I aminoacyl-tRNA synthetase family.
    Contains 1 tRNA-binding domain.
  • 细胞定位Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CMTDIC antibody
    • SYYC_HUMAN antibody
    • Tyrosine tRNA ligase, cytoplasmic antibody
    • Tyrosine tRNA ligase 1, cytoplasmic antibody
    • Tyrosyl tRNA synthetase antibody
    • Tyrosyl--tRNA ligase antibody
    • Tyrosyl-tRNA synthetase, cytoplasmic antibody
    • TyrRS antibody
    • yars antibody
    • YRS antibody
    • YTS antibody
    see all

Anti-Tyrosyl tRNA synthetase antibody [YAR5H08] 图像

  • Anti-Tyrosyl tRNA synthetase antibody [YAR5H08] (ab50961) at 1/100 dilution + HeLa whole cell lysate at 50 µg

    Secondary
    anti-mouse IgG at 1/2500 dilution
    Developed using the ECL technique

    Predicted band size : 58 kDa
    Observed band size : 58 kDa
  • Immunoprecipititation analysis using 10ug ab50961 per 500ug RIPA HeLa cell lysate. Immunoblot using ab50961 at 1/100 dilution and TrueBlotTM anti-mouse IgG at 1/1000 as secondary antibody.
  • Anti-Tyrosyl tRNA synthetase antibody [YAR5H08] (ab50961) at 1/100 dilution + NIH3T3 whole cell lysate at 50 µg

    Secondary
    anti-mouse IgG at 1/2500 dilution
    Developed using the ECL technique

    Predicted band size : 58 kDa
    Observed band size : 58 kDa
  • ab50961 at 1/10 dilution staining human YARS in HeLa cells by Immunocytochemistry. AlexaFluor 488 Goat anti-mouse IgG at 1/200 as secondary.

Anti-Tyrosyl tRNA synthetase antibody [YAR5H08] (ab50961)参考文献

This product has been referenced in:
  • Park SJ  et al. Evaluation of Multi-tRNA Synthetase Complex by Multiple Reaction Monitoring Mass Spectrometry Coupled with Size Exclusion Chromatography. PLoS One 10:e0142253 (2015). WB ; Human . Read more (PubMed: 26544075) »

See 1 Publication for this product

Product Wall

Thank you for your inquiry.

Unfortunately, we are not able to release the immunogen sequence for this particular antibody as it is proprietary information.

We aim to provide as much information as possible to our customers, so I a...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"