ab41528 gave a positive result in the following tissue lysates: Mouse Substantia Nigra, Rat Substantia Nigra, Mouse Dopaminergic Nucleus and Rat Dopaminergic Nucleus.
This antibody gave a positive result in IHC in the following FFPE tissue: Rat 6 week brain.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Plays an important role in the physiology of adrenergic neurons.
Mainly expressed in the brain and adrenal glands.
Catecholamine biosynthesis; dopamine biosynthesis; dopamine from L-tyrosine: step 1/2.
Defects in TH are the cause of dystonia DOPA-responsive autosomal recessive (ARDRD) [MIM:605407]; also known as autosomal recessive Segawa syndrome. ARDRD is a form of DOPA-responsive dystonia presenting in infancy or early childhood. Dystonia is defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures. Some cases of ARDRD present with parkinsonian symptoms in infancy. Unlike all other forms of dystonia, it is an eminently treatable condition, due to a favorable response to L-DOPA. Note=May play a role in the pathogenesis of Parkinson disease (PD). A genome-wide copy number variation analysis has identified a 34 kilobase deletion over the TH gene in a PD patient but not in any controls.
Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.
ICC/IF image of ab41528 stained rat PC12 cells. The cells were methanol fixed (5 min), permabilised in PBS-T (20 min) and incubated with the antibody (ab41528, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
Western blot - Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (ab41528)
All lanes : Anti-Tyrosine Hydroxylase antibody - Neuronal Marker (ab41528) at 1 µg/ml
Lane 1 : Mouse Substantia Nigra at 5 µg Lane 2 : Rat Substantia Nigra at 10 µg Lane 3 : Mouse Dopaminergic Nucleus at 10 µg Lane 4 : Rat Dopaminergic Nucleus at 10 µg
Secondary All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
IHC image of Tyrosine Hydroxylase staining in Rat 6 week brain (coronal) formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab41528, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Minipigs were deeply anesthetized with a combination of midazolam and
ketamine, prior to transcardial perfusion with phosphate buffered 4%
paraformaldehyde (pH 7.4). After perfusion, the brains were removed with special care taken to preserve the optic chiasm and the median eminence. Blocks of tissue containing the hypothalami
were dissected, postfixed in the same fixative for 1 day and subsequently cryoprotected in 30% sucrose for 3–4 days, prior to freezing. 10 series of 40-mm thick
coronal (6 animals), sagittal (1 animal), and horizontal (1 animal) cryostat
sections were collected. Coronal sections for immunohistochemistry were maintained at -18°C as free-floating sections in a cryoprotectant poly-ethylene glycol solution for up to four weeks.
Immunohistochemistry was performed using the avidin-biotin method. Accordingly,
free-floating sections were
first rinsed in Tris-buffered saline (TBS; 0.05 M; pH 7.4) for 15 minutes. Incubations with
avidin (0.1%) and