Anti-Tyrosine Hydroxylase抗体(ab51191)


  • 产品名称Anti-Tyrosine Hydroxylase抗体
    参阅全部 Tyrosine Hydroxylase 一抗
  • 描述
    兔多克隆抗体to Tyrosine Hydroxylase
  • 特异性This antibody detects endogenous levels of total Tyrosine Hydroxylase protein.
  • 经测试应用适用于: IHC-FoFr, ELISA, IHC-P, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthesized non-phosphopeptide derived from Tyrosine Hydroxylase around the phosphorylation site of Serine 19 (AVSPEQ) (Human)

  • 阳性对照
    • Extracts from 3T3 cells treated with Tyrosine Hydroxylase antibody; Human brain tissue




Our Abpromise guarantee covers the use of ab51191 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-FoFr Use at an assay dependent concentration. PubMed: 20923695
ELISA 1/10000.
IHC-P Use at an assay dependent concentration.
WB 1/300 - 1/1000. Detects a band of approximately 49 kDa (predicted molecular weight: 59 kDa).


  • 功能Plays an important role in the physiology of adrenergic neurons.
  • 组织特异性Mainly expressed in the brain and adrenal glands.
  • 通路Catecholamine biosynthesis; dopamine biosynthesis; dopamine from L-tyrosine: step 1/2.
  • 疾病相关Defects in TH are the cause of dystonia DOPA-responsive autosomal recessive (ARDRD) [MIM:605407]; also known as autosomal recessive Segawa syndrome. ARDRD is a form of DOPA-responsive dystonia presenting in infancy or early childhood. Dystonia is defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures. Some cases of ARDRD present with parkinsonian symptoms in infancy. Unlike all other forms of dystonia, it is an eminently treatable condition, due to a favorable response to L-DOPA.
    Note=May play a role in the pathogenesis of Parkinson disease (PD). A genome-wide copy number variation analysis has identified a 34 kilobase deletion over the TH gene in a PD patient but not in any controls.
  • 序列相似性Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Dystonia 14 antibody
    • DYT14 antibody
    • DYT5b antibody
    • EC antibody
    • OTTHUMP00000011225 antibody
    • OTTHUMP00000011226 antibody
    • ple antibody
    • Protein Pale antibody
    • TH antibody
    • The antibody
    • TY3H_HUMAN antibody
    • TYH antibody
    • Tyrosine 3 hydroxylase antibody
    • Tyrosine 3 monooxygenase antibody
    • Tyrosine 3-hydroxylase antibody
    • Tyrosine 3-monooxygenase antibody
    • Tyrosine hydroxylase antibody
    see all

Anti-Tyrosine Hydroxylase antibody 图像

  • Immunohistochemical analysis of paraffin-embedded human brain tissue using ab51191 (1/50 dilution). Left: without immunizing peptide, Right: with immunizing peptide
  • All lanes : Anti-Tyrosine Hydroxylase antibody (ab51191) at 1/300 dilution

    Lane 1 : Extracts from 3T3 cells treated with Forskolin (40nM, 30min) with no peptide
    Lane 2 : Extracts from 3T3 cells treated with Forskolin (40nM, 30min) with peptide

    Predicted band size : 59 kDa
    Observed band size : 49 kDa (why is the actual band size different from the predicted?)

Anti-Tyrosine Hydroxylase antibody (ab51191)参考文献

This product has been referenced in:
  • Chung K  et al. Structural and molecular interrogation of intact biological systems. Nature 497:332-7 (2013). Read more (PubMed: 23575631) »
  • Cambon K  et al. Aggressive behavior during social interaction in mice is controlled by the modulation of tyrosine hydroxylase expression in the prefrontal cortex. Neuroscience 171:840-51 (2010). IHC-FoFr ; Mouse . Read more (PubMed: 20923695) »

See all 2 Publications for this product

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The blocking peptide for ab51191 has been published as Tyrosine Hydroxylase peptide (ab151686):

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Tissue lysate - whole (Brain)
Loading amount 30 µg
Specification Brain
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

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Verified customer

提交于 Apr 16 2012

Thank you for your inquiry.
We do guarantee both of these antibodies, ab51191 and ab112, to work on rat IHC-P. Therefore both should be adapted for your study. The ab112 has been used up to 1/750 in IHC-P and has been produced against the full len...

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