Anti-Tyrosine Hydroxylase抗体(ab112)

概述

  • 产品名称
    Anti-Tyrosine Hydroxylase抗体
    参阅全部 Tyrosine Hydroxylase 一抗
  • 描述
    兔多克隆抗体to Tyrosine Hydroxylase
  • 经测试应用
    适用于: IHC-Fr, IHC-P, IHC-FrFl, ICC/IF, IHC-FoFr, WB, IP, ICCmore details
  • 种属反应性
    与反应: Mouse, Rat, Goat, Cat, Human, Pig
    预测可用于: Mammal
  • 免疫原

    Full length SDS denatured protein (purified from pheochromocytoma) (Rat).

  • 常规说明

    ab112 can be used as a marker for dopaminergic and noradrenergic neurons.

性能

应用

Our Abpromise guarantee covers the use of ab112 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr 1/750 - 1/1500.
IHC-P 1/750.
IHC-FrFl 1/1000. PubMed: 222726
ICC/IF 1/1000.
IHC-FoFr Use at an assay dependent concentration. PubMed: 20357108
WB 1/200. Predicted molecular weight: 60 kDa.
IP Use at an assay dependent concentration.
ICC 1/2000.

靶标

  • 功能
    Plays an important role in the physiology of adrenergic neurons.
  • 组织特异性
    Mainly expressed in the brain and adrenal glands.
  • 通路
    Catecholamine biosynthesis; dopamine biosynthesis; dopamine from L-tyrosine: step 1/2.
  • 疾病相关
    Defects in TH are the cause of dystonia DOPA-responsive autosomal recessive (ARDRD) [MIM:605407]; also known as autosomal recessive Segawa syndrome. ARDRD is a form of DOPA-responsive dystonia presenting in infancy or early childhood. Dystonia is defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures. Some cases of ARDRD present with parkinsonian symptoms in infancy. Unlike all other forms of dystonia, it is an eminently treatable condition, due to a favorable response to L-DOPA.
    Note=May play a role in the pathogenesis of Parkinson disease (PD). A genome-wide copy number variation analysis has identified a 34 kilobase deletion over the TH gene in a PD patient but not in any controls.
  • 序列相似性
    Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Dystonia 14 antibody
    • DYT14 antibody
    • DYT5b antibody
    • EC 1.14.16.2 antibody
    • OTTHUMP00000011225 antibody
    • OTTHUMP00000011226 antibody
    • ple antibody
    • Protein Pale antibody
    • TH antibody
    • The antibody
    • TY3H_HUMAN antibody
    • TYH antibody
    • Tyrosine 3 hydroxylase antibody
    • Tyrosine 3 monooxygenase antibody
    • Tyrosine 3-hydroxylase antibody
    • Tyrosine 3-monooxygenase antibody
    • Tyrosine hydroxylase antibody
    see all

Anti-Tyrosine Hydroxylase antibody 图像

  • Paraffin embedded sections of rat brain tissue were stained for Tyrosine Hydroxylase with ab112 at 1/5000 dilution in immunohistochemical analysis (panel 1). Panel 2 shows an image in which ab112 was replaced with a Rabbit IgG1 isotype control.

  • All lanes : Anti-Tyrosine Hydroxylase antibody (ab112) at 1/200 dilution

    Lane 1 : Mouse cell lysate (CATH.a neuron)
    Lane 2 : Mouse cell lysate (CATH.a neuron)
    Lane 3 : Mouse cell lysate (CATH.a neuron)
    Lane 4 : Mouse cell lysate (CATH.a neuron)

    Lysates/proteins at 40 µg per lane.

    Secondary
    Goat Anti-Rabbit HRP
    Developed using the ECL technique

    Predicted band size : 60 kDa


    Exposure time : 50 seconds

    Image courtesy of an anonymous AbReview

  • ab112 staining tyrosine hydroxulase in mouse brain tissue. Sections were permeabilized with 0.5% Triton X-100 in PBS at room temperature for 10 minutes. Samples were incubated with primary antibody (1:500) overnight at 4C.

  • ab112 staining Tyrosine Hydroxylase in mouse brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA for 10 minutes at 21°C; antigen retrieval was by heat mediation in citric acid. Samples were incubated with primary antibody (1/800 in TBS/BSA/azide) for 2 hours at 21°C. A Biotin-conjugated goat anti-rabbit IgG polyclonal (1/250) was used as the secondary antibody. The image of Caudate putamen shows characteristic nerve fibre positivity

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  • Predicted band size : 60 kDa

    Western Blot: ab112 - WB analysis of Tyrosine Hydroxylase in PC12 cell whole lysate.

  • PFA-fixed, Triton X-100 permeabilized human neurons in cerebral organoid stained for Tyrosine Hydroxylase (green) using ab112 at 1/300 dilutionl in ICC/IF.

  • ab112 at 1/800 staining rat dopaminergic neuronal tissue sections (araldite resin sections) by immunohistochemistry. The tissue was paraformaldehyde fixed and then an antigen retrieval step was carried out (heat mediated). A biotinylated goat anti-rabbit IgG (ab6720) was used as the secondary.

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  • Predicted band size : 60 kDa

    Western Blot: ab112 - 10 ug of rat caudate lysate showing specific immunolabeling of the ~60k TH protein.

  • ab112 at 1/500 staining rat brain tissue (ab29475) sections by IHC-P. The tissue was paraformaldehyde fixed and blocked with serum prior to incubation with the antibody for 14 hours. A biotinylated swine anti-rabbit IgG was used as the secondary.

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  • ab112, at 1/750, staining tyrosine hydroxylase in mouse brain tissue (ab30151) by Immunohistochemistry (Frozen sections). Sections were PFA fixed, permeabilized in 0.3 Triton X-100 prior to blocking in 5% serum for 1 hour at 22°C and then incubated with ab112, for 16 hours at 22°C. Alexa fluor® 555 goat polyclonal to rabbit Ig (ab150078), diluted 1/1000, was used as the secondary antibody.

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  • Immunohistochemical staining of retina tissue using ab112.

Anti-Tyrosine Hydroxylase antibody (ab112)参考文献

This product has been referenced in:
  • Longo F  et al. Age-dependent dopamine transporter dysfunction and Serine129 phospho-a-synuclein overload in G2019S LRRK2 mice. Acta Neuropathol Commun 5:22 (2017). IHC-FrFl ; Mouse . Read more (PubMed: 28292328) »
  • Chen G  et al. Antidyskinetic Effects of MEK Inhibitor Are Associated with Multiple Neurochemical Alterations in the Striatum of Hemiparkinsonian Rats. Front Neurosci 11:112 (2017). IHC-P, ICC/IF ; Rat . Read more (PubMed: 28337120) »

See all 48 Publications for this product

Product Wall

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (neurons in human cerebral organoid)
Permeabilization
Yes - Triton X-100
Specification
neurons in human cerebral organoid
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 37°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Jul 12 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Pig Tissue sections (Nerve surrounding common hepatic artery)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer pH 6.0
Permeabilization
No
Specification
Nerve surrounding common hepatic artery
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

提交于 Jan 14 2017

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (brain)
Permeabilization
No
Specification
brain
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Fixative
Paraformaldehyde
Username

Mr. Tamas Bellak

Verified customer

提交于 Aug 29 2016

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Brain)
Permeabilization
Yes - Triton x-100, 0.01%
Specification
Brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Jun 18 2015

Application
Immunohistochemistry (Frozen sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Sample
Mouse Tissue sections (heart)
Specification
heart
Permeabilization
Yes - Triton x-100, 0.01%
Fixative
Paraformaldehyde
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Verified customer

提交于 Feb 13 2015

Application
Western blot
Loading amount
40 µg
Gel Running Conditions
Reduced Denaturing
Sample
Mouse Cell lysate - whole cell (CATH.a neuron)
Specification
CATH.a neuron
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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提交于 Jan 30 2015

Application
Immunocytochemistry
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1%
Sample
Mouse Cultured Cells (CATH.a neuron)
Specification
CATH.a neuron
Permeabilization
Yes - Triton x-100, 0.01%
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

提交于 Jan 16 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Sample
Mouse Tissue sections (Brain)
Specification
Brain
Permeabilization
No
Fixative
Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

提交于 Oct 06 2014

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Sample
Rat Tissue sections (Brain)
Specification
Brain
Permeabilization
No
Fixative
Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

提交于 Aug 10 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (brain cell line)
Specification
brain cell line
Fixative
Acetone
Permeabilization
No
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 21°C
Username

Abcam user community

Verified customer

提交于 Jan 31 2013

1-10 of 31 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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