概述

  • 产品名称Anti-TYRO3抗体
    参阅全部 TYRO3 一抗
  • 描述
    兔多克隆抗体to TYRO3
  • 经测试应用适用于: IHC-P, WB, ICC/IFmore details
  • 种属反应性
    与反应: Rat, Human
    预测可用于: Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 850 to the C-terminus of Human TYRO3.

    (Peptide available as ab95403.)

  • 阳性对照
    • This antibody gave a positive signal in Rat and Mouse brain tissue lysates, as well as inthe following whole cell lysates: T98G; SKNBE; SHSY5Y.

性能

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应用

Our Abpromise guarantee covers the use of ab79778 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 92 kDa (predicted molecular weight: 96 kDa).
ICC/IF Use a concentration of 5 µg/ml.

靶标

  • 功能May be involved in cell adhesion processes, particularly in the central nervous system. In case of filovirus infection, seems to function as a cell entry factor.
  • 组织特异性Abundant in the brain and lower levels in other tissues.
  • 序列相似性Belongs to the protein kinase superfamily. Tyr protein kinase family. AXL/UFO subfamily.
    Contains 2 fibronectin type-III domains.
    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.
  • 细胞定位Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Brt antibody
    • BYK antibody
    • DTK antibody
    • RSE antibody
    • SKY antibody
    • Tif antibody
    • tyro3 antibody
    • TYRO3_HUMAN antibody
    • Tyrosine-protein kinase byk antibody
    • Tyrosine-protein kinase DTK antibody
    • Tyrosine-protein kinase receptor TYRO3 antibody
    • Tyrosine-protein kinase RSE antibody
    • Tyrosine-protein kinase SKY antibody
    see all

Anti-TYRO3 antibody 图像

  • All lanes : Anti-TYRO3 antibody (ab79778) at 1 µg/ml

    Lane 1 : Brain (Mouse) Tissue Lysate
    Lane 2 : Brain (Rat) Tissue Lysate
    Lane 3 : U-87 MG (Human glioblastoma astrocytoma) Whole Cell Lysate
    Lane 4 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
    Lane 5 : SK N BE (Human neuroblastoma) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 96 kDa
    Observed band size : 92 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 36 kDa,60 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 12 minutes
  • ICC/IF image of ab79778 stained PC12 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab79778, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% Methanol fixed (5 min) PC12 cells at 5µg/ml.
  • IHC image of TYRO3 staining in Human Hippocampus FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab79778, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX

Anti-TYRO3 antibody (ab79778)参考文献

This product has been referenced in:
  • Taichman RS  et al. GAS6 receptor status is associated with dormancy and bone metastatic tumor formation. PLoS One 8:e61873 (2013). IHC-P ; Human . Read more (PubMed: 23637920) »

See 1 Publication for this product

Product Wall

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing
Sample Human Cell lysate - whole cell (gastric cancer)
Specification gastric cancer
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 27°C
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提交于 Feb 17 2014

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