概述

  • 产品名称
    Anti-TXNRD1抗体
    参阅全部 TXNRD1 一抗
  • 描述
    兔多克隆抗体to TXNRD1
  • 宿主
    Rabbit
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Cow, Dog, Pig, Chimpanzee, Macaque monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 250 - 350 of Human TXNRD1.

    参阅Abcam的专有抗源政策

  • 阳性对照
    • This antibody gave a positive signal in Human Testis and Spleen tissue lysates as well as the following whole cell lysates: HeLa; HepG2; HEK293; MCF7.

性能

应用

Our Abpromise guarantee covers the use of ab113904 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 71 kDa). Abcam recommends using milk as the blocking agent (3%).

靶标

  • 功能
    Isoform 1 may possess glutaredoxin activity as well as thioredoxin reductase activity and induces actin and tubulin polymerization, leading to formation of cell membrane protrusions. Isoform 4 enhances the transcriptional activity of estrogen receptors alpha and beta while isoform 5 enhances the transcriptional activity of the beta receptor only. Isoform 5 also mediates cell death induced by a combination of interferon-beta and retinoic acid.
  • 组织特异性
    Isoform 1 is expressed predominantly in Leydig cells (at protein level). Also expressed in ovary, spleen, heart, liver, kidney and pancreas and in a number of cancer cell lines. Isoform 4 is widely expressed with highest levels in kidney, testis, uterus, ovary, prostate, placenta and fetal liver.
  • 序列相似性
    Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.
    Contains 1 glutaredoxin domain.
  • 结构域
    The N-terminal glutaredoxin domain found in isoform 1 does not contain the C-P-Y-C redox-active motif normally found in glutaredoxins and has been found to be inactive in classical glutaredoxin assays.
  • 翻译后修饰
    The N-terminus of isoform 5 is blocked.
    ISGylated.
  • 细胞定位
    Cytoplasm and Cytoplasm. Nucleus.
  • Information by UniProt
  • 数据库链接
    • 别名
      • cytoplasmic antibody
      • Gene associated with retinoic and IFN-induced mortality 12 protein antibody
      • Gene associated with retinoic and interferon-induced mortality 12 protein antibody
      • Gene associated with retinoid IFN induced mortality 12 protein antibody
      • GRIM 12 antibody
      • GRIM-12 antibody
      • GRIM12 antibody
      • KDRF antibody
      • KM 102 derived reductase like factor antibody
      • KM-102-derived reductase-like factor antibody
      • MGC9145 antibody
      • Oxidoreductase antibody
      • Thioredoxin reductase 1 antibody
      • Thioredoxin reductase 1 cytoplasmic antibody
      • Thioredoxin reductase GRIM 12 antibody
      • Thioredoxin reductase TR1 antibody
      • TR 1 antibody
      • TR antibody
      • TR1 antibody
      • TRXR 1 antibody
      • TRXR1 antibody
      • TRXR1_HUMAN antibody
      • TXNR antibody
      • TXNRD 1 antibody
      • Txnrd1 antibody
      see all

    图片

    • Western blot - Anti-TXNRD1 antibody (ab113904)
      Western blot - Anti-TXNRD1 antibody (ab113904)
      All lanes : Anti-TXNRD1 antibody (ab113904) at 1 µg/ml (Milk blocking - 3%)

      Lane 1 : Human testis tissue lysate - total protein (ab30257)
      Lane 2 : Human spleen tissue lysate - total protein (ab29699)
      Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
      Lane 4 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
      Lane 5 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
      Lane 6 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 71 kDa
      Observed band size: 60 kDa (why is the actual band size different from the predicted?)
      Additional bands at: 150 kDa, 37 kDa, 65 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 8 minutes


      The predicted molecular weight of TXNRD1 is 71 kDa (SwissProt), however we expect to observe a banding pattern around 60 kDa. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.

    实验方案

    文献

    ab113904 has not yet been referenced specifically in any publications.

    发表研究结果有使用 ab113904？请让我们知道，以便我们可以引用本数据表中的参考文章。

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