重组Anti-TXNIP抗体[EPR14774] -小鼠IgG2b (Chimeric) - BSA and Azide free (ab232330)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [EPR14774] to TXNIP - Chimeric – BSA and Azide free
- Suitable for: Flow Cyt (Intra), WB, ICC/IF, IHC-P
- Reacts with: Mouse, Rat, Human
概述
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产品名称
Anti-TXNIP抗体[EPR14774] -小鼠IgG2b (Chimeric) - BSA and Azide free
参阅全部 TXNIP 一抗 -
描述
小鼠单克隆抗体[EPR14774] to TXNIP - Chimeric – BSA and Azide free -
宿主
Mouse -
经测试应用
适用于: Flow Cyt (Intra), WB, ICC/IF, IHC-Pmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- IHC-P: Human kidney and Mouse liver tissue. WB: HeLa, 293T, THP-1, MDA-MB-231, BxPC-3, HUVEC, BxPC-3, PC-12 and NIH/3T3; Human liver, skeletal muscle and kidney tissue lysates
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常规说明
ab232330 is the carrier-free version of ab210826.
This mouse monoclonal chimeric antibody has been engineered from a RabMAb parent antibody (ab188865). By necessity, some rabbit sequence is retained as part of the variable domain. When multiplexing with other rabbit-derived antibodies, using cross absorbed Fc-reactive secondary antibodies are recommended.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Constituent: PBS -
无载体
是 -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR14774 -
同种型
IgG -
轻链类型
kappa -
研究领域
相关产品
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Alternative Versions
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Conjugation kits
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Isotype control
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab232330于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Flow Cyt (Intra) |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration. Detects a band of approximately 55 kDa (predicted molecular weight: 44 kDa).
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ICC/IF |
Use at an assay dependent concentration.
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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说明 |
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Flow Cyt (Intra)
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Detects a band of approximately 55 kDa (predicted molecular weight: 44 kDa). |
ICC/IF
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
May act as an oxidative stress mediator by inhibiting thioredoxin activity or by limiting its bioavailability. Interacts with COPS5 and restores COPS5-induced suppression of CDKN1B stability, blocking the COPS5-mediated translocation of CDKN1B from the nucleus to the cytoplasm. Functions as a transcriptional repressor, possibly by acting as a bridge molecule between transcription factors and corepressor complexes, and over-expression will induce G0/G1 cell cycle arrest. Required for the maturation of natural killer cells. -
序列相似性
Belongs to the arrestin family. -
翻译后修饰
Ubiquitinated; undergoes polyubiquitination catalyzed by ITCH resulting in proteasomal degradation. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 10628 Human
- Entrez Gene: 56338 Mouse
- Entrez Gene: 117514 Rat
- Omim: 606599 Human
- SwissProt: Q9H3M7 Human
- SwissProt: Q8BG60 Mouse
- SwissProt: Q5M7W1 Rat
- Unigene: 533977 Human
see all -
别名
- EST01027 antibody
- HHCPA78 antibody
- THIF antibody
see all
图片
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Ab210826 staining TXNIP in 293T (human embryonic kidney epithelial cell line) cells by Immunocytochemistry/Immunofluorescence (ICC/IF). The cells were fixed with 100% Methanol. Samples were incubated with primary antibody at 10µg/ml (1:100 dilution). An Alexa Fluor® 488 Goat Anti-Mouse was used as the secondary antibody at 2µg/ml (ab150113). Ab179504, Anti-beta IV Tubulin was used as a counterstain at 2µg/ml and ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) was used as secondary antibody counterstain at 4µg/ml.
For negative control 1, primary antibody was used at a 10µg/ml and ab150080 was used as secondary antibody at 4 µg/ml. For negative control 2, ab179504 was used as a primary antibody at 2µg/ml and ab150113 was used as a secondary antibody at 2 µg/ml. DAPI was used as a nuclear counterstain. Confocal image showing cytoplasmic staining in 293T cells.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab210826).
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Ab210826 staining TXNIP in HeLa (Human cervix adenocarcinoma epithelial cell line) by intracellular flow cytometry. Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. The sample was incubated with primary antibody at 1/100 dilution (1µg/ml) (red). An Alexa Fluor® 488 Goat anti mouse IgG (ab150113) was used at 1/2000 dilution. Rabbit monoclonal IgG (ab172730) was used as isotype control (black). Cell without incubation with primary antibody and secondary antibody (blue).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab210826).
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IHC image of ab210826 staining in a section of formalin fixed, paraffin embedded mouse normal kidney, using MOM detection kit, ab127055. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with ab210826, 2µg/ml, for 15 mins at room temperature. DAB was used as the chromogen (ab103723), diluted 1/100 and incubated for 10min at room temperature. The section was counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab210826).
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All lanes : Anti-TXNIP antibody [EPR14774] - Mouse IgG2b (Chimeric) - BSA and Azide free (ab232330) at 1/1000 dilution
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
Lane 2 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates
Lane 3 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysates
Lane 4 : PC-12 (Rat adrenal gland pheochromocyteoma) whole cell lysates
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Rabbit polyclonal secondary antibody to mouse IgG – H&L (HRP) at 1/2000 dilution
Predicted band size: 44 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
Exposure time: 30 secondsBlocking and dilution buffer: 5% NFDM/TBST.
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IHC image of TxNIP staining in a section of formalin fixed, paraffin embedded human normal kidney tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab210826, 2µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab210826).
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ab210826 stained in NIH3T3 cells. The cells were fixed with 100% methanol (5min) at room temperature and incubated with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% triton for 1h at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab210826 at 5µg/ml overnight at +4°C. The secondary antibody was ab150177 used at 1 ug/ml for 1hour at room temperature (colored green). ab206369 (Rabbit monoclonal [EPR16774] to beta Tubulin Alexa Fluor® 594) was used as a counterstaining at a 1/200 dilution for 1hour at room temperature (pseudo-colored red). DAPI was used to stain the cell nuclei (colored blue) at a concentration of 1.43µM for 1hour at room temperature.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab210826).
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ab210826 stained in HeLa cells. The cells were fixed with 100% methanol (5min) at room temperature and incubated with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% triton for 1h at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab210826 at 5µg/ml overnight at +4°C. The secondary antibody was ab150177 used at 1 ug/ml for 1hour at room temperature (colored green). ab206369 (Rabbit monoclonal [EPR16774] to beta Tubulin Alexa Fluor® 594) was used as a counterstaining at a 1/200 dilution for 1hour at room temperature (pseudo-colored red). DAPI was used to stain the cell nuclei (colored blue) at a concentration of 1.43µM for 1hour at room temperature.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab210826).
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (2)
ab232330 被引用在 2 文献中.
- Hu Y et al. Genetic landscape and autoimmunity of monocytes in developing Vogt-Koyanagi-Harada disease. Proc Natl Acad Sci U S A 117:25712-25721 (2020). PubMed: 32989127
- Yu Y et al. Bixin Attenuates Experimental Autoimmune Encephalomyelitis by Suppressing TXNIP/NLRP3 Inflammasome Activity and Activating NRF2 Signaling. Front Immunol 11:593368 (2020). PubMed: 33362775