Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human pineal tissue labelling TXNDC13 with ab82796 at 1/100. A Cy3-conjugated donkey anti-rabbit IgG (1/200) was used as the secondary antibody. Positive staining shown in the cytoplasm of cell bodies and processes of pinealocytes. Magnification: 20X. Exposure time: 0.5 - 2.0 seconds. Left - DAPI. Middle - TXNDC13. Right - Merge.
Western blot - TXNDC13 antibody (ab82796)
Anti-TXNDC13 antibody (ab82796) at 1 µg/ml + 293T cell lysate at 10 µg
Secondary HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 39 kDa Observed band size : 39 kDa Additional bands at : 90 kDa. We are unsure as to the identity of these extra bands.Gel concentration: 12%