概述

  • 产品名称Anti-TXK抗体
    参阅全部 TXK 一抗
  • 描述
    小鼠单克隆抗体to TXK
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment: LIPSNYVTEN KITNLEIYEW YHRNITRNQA EHLLRQESKE GAFIVRDSRH LGSYTISVFM GARRSTEAAI KHYQIKKNDS GQWYVAERHA FQSIPELIWY , corresponding to amino acids 131-231 of Human TXK

性能

应用

Our Abpromise guarantee covers the use of ab57674 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
  • 应用说明WB: Use at a concentration of 1-5 µg/ml.

    This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Non-receptor tyrosine kinase that plays a redundant role with ITK in regulation of the adaptive immune response. Regulates the development, function and differentiation of conventional T-cells and nonconventional NKT-cells. When antigen presenting cells (APC) activate T-cell receptor (TCR), a series of phosphorylation lead to the recruitment of TXK to the cell membrane, where it is phosphorylated at Tyr-420. Phosphorylation leads to TXK full activation. Contributes also to signaling from many receptors and participates in multiple downstream pathways, including regulation of the actin cytoskeleton. Like ITK, can phosphorylate PLCG1, leading to its localization in lipid rafts and activation, followed by subsequent cleavage of its substrates. In turn, the endoplasmic reticulum releases calcium in the cytoplasm and the nuclear activator of activated T-cells (NFAT) translocates into the nucleus to perform its transcriptional duty. With PARP1 and EEF1A1, TXK forms a complex that acts as a T-helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFNG to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production. Phosphorylates both PARP1 and EEF1A1. Phosphorylates also key sites in LCP2 leading to the up-regulation of Th1 preferred cytokine IL-2. Phosphorylates 'Tyr-201' of CTLA4 which leads to the association of PI-3 kinase with the CTLA4 receptor.
    • 组织特异性Expressed in T-cells and some myeloid cell lines. Expressed in Th1/Th0 cells with IFN-gamma-producing potential.
    • 序列相似性Belongs to the protein kinase superfamily. Tyr protein kinase family. TEC subfamily.
      Contains 1 protein kinase domain.
      Contains 1 SH2 domain.
      Contains 1 SH3 domain.
    • 翻译后修饰Phosphorylated at Tyr-420 by FYN. Autophosphorylation at Tyr-91 is critical for the activation of TXK, leading to the up-regulation of IFN-gamma gene transcription.
      The cysteine string at the N-terminus is palmitoylated and required for the proper subcellular location.
    • 细胞定位Cytoplasm. Nucleus. Cell membrane. Localizes in the vicinity of cell surface receptors in the plasma membrane after receptor stimulation. Translocates into the nucleus and enhances IFN-gamma gene transcription in T-cells.
    • Information by UniProt
    • 数据库链接
    • 别名
      • BTKL antibody
      • EC 2.7.10.2 antibody
      • MGC22473 antibody
      • Protein-tyrosine kinase 4 antibody
      • PSCTK5 antibody
      • PTK4 antibody
      • Resting lymphocyte kinase antibody
      • RLK antibody
      • TKL antibody
      • TXK antibody
      • TXK tyrosine kinase antibody
      • TXK_HUMAN antibody
      • Tyrosine protein kinase TXK antibody
      • Tyrosine-protein kinase TXK antibody
      see all

    Anti-TXK antibody 图像

    • Western blot against tagged recombinant protein immunogen using ab57674 TXK antibody at 1ug/ml. Predicted band size of immunogen is 37 kDa

    Anti-TXK antibody (ab57674)参考文献

    ab57674 has not yet been referenced specifically in any publications.

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