Anti-Twist抗体[Twist2C1a] - ChIP Grade (ab50887)

概述

  • 产品名称Anti-Twist抗体[Twist2C1a] - ChIP Grade
    参阅全部 Twist 一抗
  • 描述
    小鼠单克隆抗体[Twist2C1a] to Twist - ChIP Grade
  • 特异性Good controls are NIH3T3 and A2058 cell lysates. Given that the immunogen shares 92% homology with Twist2, it is possible that it may cross-react. Although some customers have obtained good results in IHC, we do not recommend this antibody for use in IHC.
  • 经测试应用适用于: IP, WB, Dot blot, ChIP, ICC/IF, ICCmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Recombinant fragment (Human)

  • 阳性对照

性能

应用

Our Abpromise guarantee covers the use of ab50887 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP Use at an assay dependent concentration.
WB 1/50. Predicted molecular weight: 21 kDa.

Abcam recommends using BSA blocking.

Dot blot Use at an assay dependent concentration.
ChIP Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.

靶标

  • 功能Acts as a transcriptional regulator. Inhibits myogenesis by sequestrating E proteins, inhibiting trans-activation by MEF2, and inhibiting DNA-binding by MYOD1 through physical interaction. This interaction probably involves the basic domains of both proteins. Also represses expression of proinflammatory cytokines such as TNFA and IL1B. Regulates cranial suture patterning and fusion. Activates transcription as a heterodimer with E proteins. Regulates gene expression differentially, depending on dimer composition. Homodimers induce expression of FGFR2 and POSTN while heterodimers repress FGFR2 and POSTN expression and induce THBS1 expression. Heterodimerization is also required for osteoblast differentiation.
  • 组织特异性Subset of mesodermal cells.
  • 疾病相关Defects in TWIST1 are a cause of Saethre-Chotzen syndrome (SCS) [MIM:101400]; also known as acrocephalosyndactyly type 3 (ACS3). SCS is a craniosynostosis syndrome characterized by coronal synostosis, brachycephaly, low frontal hairline, facial asymmetry, hypertelorism, broad halluces, and clinodactyly.
    Defects in TWIST1 are the cause of Robinow-Sorauf syndrome (RSS) [MIM:180750]; also known as craniosynostosis-bifid hallux syndrome. RSS is an autosomal dominant defect characterized by minor skull and limb anomalies which is very similar to Saethre-Chotzen syndrome.
    Defects in TWIST1 are the cause of craniosynostosis type 1 (CRS1) [MIM:123100]. Craniosynostosis consists of premature fusion of one or more cranial sutures, resulting in an abnormal head shape.
  • 序列相似性Contains 1 basic helix-loop-helix (bHLH) domain.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ACS3 antibody
    • B-HLH DNA binding protein antibody
    • bHLHa38 antibody
    • BPES2 antibody
    • BPES3 antibody
    • Class A basic helix-loop-helix protein 38 antibody
    • CRS1 antibody
    • H-twist antibody
    • OTTHUMP00000116043 antibody
    • SCS antibody
    • TWIST antibody
    • Twist basic helix loop helix transcription factor 1 antibody
    • Twist homolog 1 (Drosophila) antibody
    • Twist homolog 1 antibody
    • TWIST homolog of drosophila antibody
    • Twist related protein 1 antibody
    • Twist-related protein 1 antibody
    • TWIST1 antibody
    • TWST1_HUMAN antibody
    see all

Anti-Twist antibody [Twist2C1a] - ChIP Grade 图像



  • Predicted band size : 21 kDa
    Observed band size : 25 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 100 kDa,65 kDa,75 kDa. We are unsure as to the identity of these extra bands.

    This image is courtesy of an Abreview submitted by Pawel Mazur

    All lanes: Twist antibody [Twist2C1a] - ChIP Grade (ab50887) at 1/250 dilution in PBST for 8 hours at 4°C

    Lanes 2, 3, 5, 6, 8 & 9: Whole cell lysate of mouse pancreatic tumor
    Lanes 1, 4 & 7: Whole cell lysate of Mouse metastatic pancreatic carcinoma

    Lysates at 50 µg per lane

    Secondary Antibody: An HRP-conjugated sheep anti-mouse IgG polyclonal (1/2000) developed using the ECL technique

    Blocking Step: 10% Milk for 1 hour at room temperature

    See Abreview

  • Immunostaining with anti-Twist1 monoclonal antibody ab50887 (green, 1/50 dilution) of cryosectioned wild type (fl/wt) (B, C, B′, C′) and conditional knockout (CKO) (D, E, D′, E′) mouse embyonic day 11.5 forelimb buds of embryos harvested from mothers injected with tamoxifen at embryonic day 10.5. (C, C′, E, E′) Merged images showing counterstaining with DAPI (blue). (B′–E′) Higher magnification images of the boxed regions in B–E. An AlexaFlour 488 conjugated donkey anti-mouse was used as the secondary antibody

  • ChIP analysis using ab50887 binding Twist in Human primary macrophages. Cells were cross-linked for 15 minutes with formaldehyde. Samples were incubated with primary antibody  (1/100) for 12 hours at 4°C. Protein binding was detected using real-time PCR.

    See Abreview

  • Ab50887 at 1/1 dilution staining HeLa cells; visualised with AlexaFluor®488 Goat Anti-mouse IgG at 1/200 dilution
  • Lane 1 :
    Lanes 2 - 5 : Anti-Twist antibody [Twist2C1a] - ChIP Grade (ab50887) at 1/50 dilution

    Lane 1 : Marker lane
    Lane 2 : Hela whole cell lysate, 25µg
    Lane 3 : A2058 whole cell lysate, 25µg
    Lane 4 : NIH3T3 whole cell lysate, 25µg
    Lane 5 : F2408 whole cell lysate, 25µg


    Predicted band size : 21 kDa
    Observed band size : 25 kDa (why is the actual band size different from the predicted?)


    Exposure time : 2 minutes
    SDS-PAGE with 10-20% gradient gel.
  • ab50887 staining Twist in a Rat choroid plexus cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Triton X-100 0.1% in PBS and blocked with 0.5% BSA for 30 minutes at room temperature. Samples were incubated with primary antibody (1/50 in PBS + 0.5% BSA) for 16 hours at 4°C. A FITC-conjugated Goat anti-mouse IgG polyclonal (1/80) was used as the secondary antibody.

    See Abreview

Anti-Twist antibody [Twist2C1a] - ChIP Grade (ab50887)参考文献

This product has been referenced in:
  • Wu SY  et al. A miR-192-EGR1-HOXB9 regulatory network controls the angiogenic switch in cancer. Nat Commun 7:11169 (2016). ChIP ; Human . Read more (PubMed: 27041221) »
  • Bouard C  et al. Deciphering the molecular mechanisms underlying the binding of the TWIST1/E12 complex to regulatory E-box sequences. Nucleic Acids Res 44:5470-89 (2016). WB ; Human . Read more (PubMed: 27151200) »

See all 60 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (orthotopic tumor tumor)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Low pH
Permeabilization Yes - TritonX100 0.025% 10min RT
Specification orthotopic tumor tumor
Blocking step MOM kit as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Fixative Paraformaldehyde
Username

Dr. Clara Lubeseder-Martellato

Verified customer

提交于 Jun 13 2016

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Permeabilization Yes - 0.5% TritonX100 in PBS
Specification HeLa
Fixative Paraformaldehyde
Username

Dr. Kirk McManus

Verified customer

提交于 Aug 07 2015

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Sample Human Cell (HEK 293T)
Specification HEK 293T
Permeabilization Yes - Triton 1%
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Nov 18 2014

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate pH6
Sample Dog Tissue sections (MDCK pellets in paraffin)
Specification MDCK pellets in paraffin
Permeabilization Yes - Tween-20
Fixative Formaldehyde
Username

Abcam user community

Verified customer

提交于 Nov 04 2014

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Sample Human Cell (skin)
Specification skin
Permeabilization No
Fixative Formaldehyde
Username

Ms. Eunhye Kang

Verified customer

提交于 Jun 12 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Sample Human Cell (oral squamous cell carcinoma)
Specification oral squamous cell carcinoma
Permeabilization No
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Sep 10 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris based pH 9.5
Sample Human Tissue sections (oral cavity)
Specification oral cavity
Permeabilization No
Fixative Formaldehyde
Username

Abcam user community

Verified customer

提交于 Sep 05 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 1e+006 cells
Gel Running Conditions Reduced Denaturing
Sample Human Cell lysate - whole cell (primary humanmacrophages)
Specification primary humanmacrophages
Treatment MDP 100ug/ml
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Jun 24 2013

Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing (4-20%)
Sample Human Cell lysate - whole cell (Different Cell types)
Specification Different Cell types
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

Verified customer

提交于 Jun 10 2013

Thank you for taking time to complete our questionnaire. I am sorry to hear that this antibody is not providing satisfactory results.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"