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Our Abpromise guarantee covers the use of ab49254 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 2.5 µg/ml. Detects a band of approximately 22 kDa (predicted molecular weight: 21 kDa).Can be blocked with Human Twist peptide (ab111667).
Abcam recommends blocking with BSA.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"