重组
RabMAb

Anti-TWEAKR抗体[EPR3179] (ab109365)

概述

  • 产品名称
    Anti-TWEAKR抗体[EPR3179]
    参阅全部 TWEAKR 一抗
  • 描述
    兔单克隆抗体[EPR3179] to TWEAKR
  • 经测试应用
    适用于: IHC-Fr, WB, IP, IHC-Pmore details
    不适用于: ICC
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human TWEAKR (internal sequence).

  • 阳性对照
    • HT-29, NIH/3T3, L6, C2C12, HUVEC and HT-1080 cell lysates; Human uterus tissue, human bladder carcinoma and human cardiac muscle.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

性能

应用

Our Abpromise guarantee covers the use of ab109365 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr Use at an assay dependent concentration. PubMed: 24467773
WB 1/1000 - 1/10000. Predicted molecular weight: 14 kDa.
IP 1/40.

For unpurified use at 1/10.

IHC-P 1/50 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

See protocols (link: http://www.abcam.com/protocols/ihc-antigen-retrieval-protocol).

  • 应用说明
    Is unsuitable for ICC.
  • 靶标

    • 功能
      Receptor for TNFSF12/TWEAK. Weak inducer of apoptosis in some cell types. Promotes angiogenesis and the proliferation of endothelial cells. May modulate cellular adhesion to matrix proteins.
    • 组织特异性
      Highly expressed in heart, placenta and kidney. Intermediate expression in lung, skeletal muscle and pancreas.
    • 序列相似性
      Contains 1 TNFR-Cys repeat.
    • 细胞定位
      Membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • CD 266 antibody
      • CD266 antibody
      • CD266 antigen antibody
      • FGF inducible 14 antibody
      • FGF-inducible 14 antibody
      • Fibroblast growth factor inducible immediate early response protein 14 antibody
      • Fibroblast growth factor-inducible immediate-early response protein 14 antibody
      • FN 14 antibody
      • FN14 antibody
      • TNFRSF 12A antibody
      • TNFRSF12A antibody
      • TNR12_HUMAN antibody
      • Tumor necrosis factor receptor superfamily member 12A antibody
      • TWEAK R antibody
      • Tweak receptor antibody
      • Tweak-receptor antibody
      • TweakR antibody
      see all

    图片

    • ab109365, at 1/50 dilution, staining TWEAKR in Human uterus tissue by Immunohistochemistry.
    • Immunohistochemical analysis of paraffin-embedded human bladder carcinoma sections labelling TWEAKR with purified ab109365 at dilution of 1/100. The secondary antibody used was ab97051; a goat anti-rabbit IgG H&L (HRP) at dilution of 1/500. The sample was counterstained with hematoxylin. Antigen retrieval was performed using EDTA Buffer; pH 9.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset.

    • Immunohistochemical analysis of paraffin-embedded human cardiac muscle sections labelling TWEAKR with purified ab109365 at dilution of 1/100. The secondary antibody used was ab97051; a goat anti-rabbit IgG H&L (HRP) at dilution of 1/500. The sample was counterstained with hematoxylin. Antigen retrieval was performed using EDTA Buffer; pH 9.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset.

    • All lanes : Anti-TWEAKR antibody [EPR3179] (ab109365) at 1/1000 dilution

      Lane 1 : HUVEC (human umbilical vein/vascular endothelium) whole cell lysate
      Lane 2 : HT-1080 (human fibrosarcoma) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size : 14 kDa
      Observed band size : 14-17 kDa (why is the actual band size different from the predicted?)
      Blocking/Diluting buffer 5% NFDM /TBST
    • All lanes : Anti-TWEAKR antibody [EPR3179] (ab109365) at 1/1000 dilution

      Lane 1 : NIH/3T3 (mouse embryo) whole cell lysate
      Lane 2 : L6 (rat skeletal muscle) whole cell lysate
      Lane 3 : C2C12 (mouse muscle) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size : 14 kDa
      Observed band size : 14-17 kDa (why is the actual band size different from the predicted?)
      Blocking/Diluting buffer 5% NFDM /TBST
    • ab109365 at 1/40 immunoprecipitating TWEAKR in HUVEC (human cervix adenocarcinoma) whole cell lysate observed at 14 – 17 KDa (lanes 1 and 2).

      Lane 1 (input): HUVEC whole cell lysate 10ug

      Lane 2 (+): ab109365 + HUVEC whole cell lysate.

      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab109365 in HUVEC whole cell lysate

      ab131366 VeriBlot for IP (HRP) was used as the secondary antibody (1/10 000) was used as the secondary antibody.

      Blocking/Diluting buffer and concentration: 5% NFDM/TBST.

    • All lanes : Anti-TWEAKR antibody [EPR3179] (ab109365) at 1/1000 dilution

      Lane 1 : HT-29 cell lysate
      Lane 2 : HUVEC cell lysate
      Lane 3 : HT-1080 cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 14 kDa

    文献

    This product has been referenced in:
    • Liu Y  et al. TWEAK/Fn14 Activation Participates in Ro52-Mediated Photosensitization in Cutaneous Lupus Erythematosus. Front Immunol 8:651 (2017). IHC-P, Flow Cyt ; Human . Read more (PubMed: 28620393) »
    • Niu N  et al. RNA-binding protein SAMD4 regulates skeleton development through translational inhibition of Mig6 expression. Cell Discov 3:16050 (2017). Read more (PubMed: 28163927) »

    See all 8 Publications for this product

    客户评价及客户问答

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (skin tumor)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 10mM Sodium Citrate buffer pH6
    Permeabilization
    No
    Specification
    skin tumor
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Fixative
    10% normal buffered formalin
    Username

    Abcam user community

    Verified customer

    提交于 Jul 16 2016

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (kidney)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 10mM Citrate buffer pH6
    Permeabilization
    No
    Specification
    kidney
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Fixative
    10% buffered normal formalin
    Username

    Abcam user community

    Verified customer

    提交于 Jul 16 2016

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (bone)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 10mM sodium citrate pH6
    Permeabilization
    No
    Specification
    bone
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Fixative
    10% normal buffered formalin
    Username

    Abcam user community

    Verified customer

    提交于 Jul 16 2016

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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