Anti-TWEAKR抗体(ab85089)

概述

  • 产品名称
  • 描述
    兔多克隆抗体to TWEAKR
  • 经测试应用
    适用于: ICC/IF, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    A peptide from the intermediate residues of Human TWEAKR protein, NM_016639.1

  • 阳性对照
    • liver

性能

  • 形式
    Lyophilised:Reconstitute with 200ul distilled sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.02% Sodium Azide
    Constituents: 2% BSA
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab85089 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
IHC-P 1/50 - 1/200.

靶标

  • 功能
    Receptor for TNFSF12/TWEAK. Weak inducer of apoptosis in some cell types. Promotes angiogenesis and the proliferation of endothelial cells. May modulate cellular adhesion to matrix proteins.
  • 组织特异性
    Highly expressed in heart, placenta and kidney. Intermediate expression in lung, skeletal muscle and pancreas.
  • 序列相似性
    Contains 1 TNFR-Cys repeat.
  • 细胞定位
    Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CD 266 antibody
    • CD266 antibody
    • CD266 antigen antibody
    • FGF inducible 14 antibody
    • FGF-inducible 14 antibody
    • Fibroblast growth factor inducible immediate early response protein 14 antibody
    • Fibroblast growth factor-inducible immediate-early response protein 14 antibody
    • FN 14 antibody
    • FN14 antibody
    • TNFRSF 12A antibody
    • TNFRSF12A antibody
    • TNR12_HUMAN antibody
    • Tumor necrosis factor receptor superfamily member 12A antibody
    • TWEAK R antibody
    • Tweak receptor antibody
    • Tweak-receptor antibody
    • TweakR antibody
    see all

图片

  • Immunohistochemical analysis of TWEAKR expression in paraffin embedded formalin fixed liver using 1/100 ab85089. Note membrane staining.
  • ICC/IF image of ab85089 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab85089, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

文献

ab85089 has not yet been referenced specifically in any publications.

客户评价及客户问答

Thank you for your kind email. I am sorry to hear that the antibody is still giving you problems. Before sending the free of charge replacement, I just wanted to confirm that it is ab109365 you would like to receive, right? http://www.abcam.com/tw...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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