概述

  • 产品名称Anti-TWEAKR抗体
    参阅全部 TWEAKR 一抗
  • 描述
    兔多克隆抗体to TWEAKR
  • 特异性This antibody reacts with TWEAKR
  • 经测试应用适用于: ICC/IF, WB, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Human IgG1 Fc-tagged TWEAKR protein

  • 阳性对照
    • HepG2 cell lysate, 293 cells and human liver tissue

性能

应用

Our Abpromise guarantee covers the use of ab62496 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 10 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 33 kDa (predicted molecular weight: 14 kDa).
ELISA Use at an assay dependent concentration.
IHC-P Use a concentration of 2.5 µg/ml.

靶标

  • 功能Receptor for TNFSF12/TWEAK. Weak inducer of apoptosis in some cell types. Promotes angiogenesis and the proliferation of endothelial cells. May modulate cellular adhesion to matrix proteins.
  • 组织特异性Highly expressed in heart, placenta and kidney. Intermediate expression in lung, skeletal muscle and pancreas.
  • 序列相似性Contains 1 TNFR-Cys repeat.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CD 266 antibody
    • CD266 antibody
    • CD266 antigen antibody
    • FGF inducible 14 antibody
    • FGF-inducible 14 antibody
    • Fibroblast growth factor inducible immediate early response protein 14 antibody
    • Fibroblast growth factor-inducible immediate-early response protein 14 antibody
    • FN 14 antibody
    • FN14 antibody
    • TNFRSF 12A antibody
    • TNFRSF12A antibody
    • TNR12_HUMAN antibody
    • Tumor necrosis factor receptor superfamily member 12A antibody
    • TWEAK R antibody
    • Tweak receptor antibody
    • Tweak-receptor antibody
    • TweakR antibody
    see all

Anti-TWEAKR antibody 图像

  • All lanes : Anti-TWEAKR antibody (ab62496) at 1 µg/ml

    Lane 1 : mock transfected 293 cells
    Lane 2 : Fn14-GPI transfected 293 cells

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 14 kDa
    Observed band size : 33 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemistry of TWEAKR in human liver tissue with ab62496 antibody at 2.5 µg/ml.


  • Predicted band size : 14 kDa
  • ICC/IF image of ab62496 stained HeLa cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab62496, 10µg/ml) overnight at +4°C. The secondary antibody (green) was for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
  • Immunofluorescence of Fn14 in Human Liver cells using ab62496 at 20 ug/ml.

Anti-TWEAKR antibody (ab62496)参考文献

This product has been referenced in:
  • Affò S  et al. Transcriptome analysis identifies TNF superfamily receptors as potential therapeutic targets in alcoholic hepatitis. Gut : (2012). IHC-P ; Human . Read more (PubMed: 22637703) »

See 1 Publication for this product

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