概述

  • 产品名称
  • 描述
    兔多克隆抗体to TWEAK
  • 经测试应用
    适用于: IHC-P, WB, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Human, Pig
  • 免疫原

    Recombinant full length TWEAK (Human)

  • 阳性对照
    • HT29 (Human colon adenocarcinoma grade II cell line) whole cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab37170 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 10 µg/ml.
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 27 kDa). TWEAK may migrate at aberrant poitions in SDS-PAGE.
ICC/IF Use a concentration of 10 µg/ml.

靶标

  • 功能
    Binds to FN14 and possibly also to TNRFSF12/APO3. Weak inducer of apoptosis in some cell types. Mediates NF-kappa-B activation. Promotes angiogenesis and the proliferation of endothelial cells. Also involved in induction of inflammatory cytokines.
  • 组织特异性
    Highly expressed in adult heart, pancreas, skeletal muscle, brain, colon, small intestine, lung, ovary, prostate, spleen, lymph node, appendix and peripheral blood lymphocytes. Low expression in kidney, testis, liver, placenta, thymus and bone marrow. Also detected in fetal kidney, liver, lung and brain.
  • 序列相似性
    Belongs to the tumor necrosis factor family.
  • 翻译后修饰
    The soluble form derives from the membrane form by proteolytic processing.
  • 细胞定位
    Secreted and Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • APO 3 ligand antibody
    • APO 3L antibody
    • APO3 ligand antibody
    • APO3/DR3 ligand antibody
    • APO3L antibody
    • DR3LG antibody
    • MGC129581 antibody
    • MGC20669 antibody
    • secreted form antibody
    • TNF-related weak inducer of apoptosis antibody
    • TNF12_HUMAN antibody
    • TNFSF 12 antibody
    • Tnfsf12 antibody
    • TNFSF12 protein antibody
    • Tumor necrosis factor (ligand) superfamily member 12 antibody
    • Tumor necrosis factor ligand superfamily member 12 antibody
    • Tumor necrosis factor superfamily member 12 antibody
    • TWEAK antibody
    • UNQ181/PRO207 antibody
    see all

图片



  • Predicted band size : 27 kDa
    Western Blot of HeLa cell lysate labeling TWEAK with Anti-TWEAK antibody (ab37170) at (1) 1 and (2) 2µg/ml.
  • All lanes : Anti-TWEAK antibody (ab37170) at 1 µg/ml

    Lane 1 : Recombinant human TWEAK at 0.005 µg
    Lane 2 : Recombinant human TWEAK at 0.025 µg
    Lane 3 : Recombinant human TWEAK at 0.05 µg


    Predicted band size : 27 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)
    TWEAK migrates at aberrant positions in SDS-PAGE; hence the observed band is different from the predicted molecular weight of 27 kDa.
  • ICC/IF image of ab37170 stained SKNSH cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab37170, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

文献

This product has been referenced in:
  • Qi X  et al. Lipopolysaccharide Upregulated Intestinal Epithelial Cell Expression of Fn14 and Activation of Fn14 Signaling Amplify Intestinal TLR4-Mediated Inflammation. Front Cell Infect Microbiol 7:315 (2017). WB, ICC/IF ; Pig . Read more (PubMed: 28744451) »
  • Padrão AI  et al. Endurance training prevents TWEAK but not myostatin-mediated cardiac remodelling in cancer cachexia. Arch Biochem Biophys 567:13-21 (2015). Read more (PubMed: 25575785) »

See all 4 Publications for this product

客户评价及客户问答

Thank you for sending along the western blot images. I just have a few additional questions: 1. Are the samples of TWEAK transfected cell extracts or are these samples purified recombinant TWEAK protein or are they untransfected cell lysa...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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