概述

  • 产品名称Anti-TUT1抗体
    参阅全部 TUT1 一抗
  • 描述
    兔多克隆抗体to TUT1
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • 免疫原

    Synthetic peptide corresponding to a region within N terminal amino acids 215-264 (DLDLFLDLGD LEEPQPVPKA PESPSLDSAL ASPLDPQALA CTPASPPDSQ) of human TUT1 (NP_073741).

  • 阳性对照
    • 293T cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab86161 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 94 kDa (predicted molecular weight: 94 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能Poly(A) polymerase that creates the 3'-poly(A) tail of specific pre-mRNAs. Localizes to nuclear speckles together with PIP5K1A and mediates polyadenylation of a select set of mRNAs, such as HMOX1. In addition to polyadenylation, it is also required for the 3'-end cleavage of pre-mRNAs: binds to the 3'UTR of targeted pre-mRNAs and promotes the recruitment and assembly of the CPSF complex on the 3'UTR of pre-mRNAs. In addition to adenylyltransferase activity, also has uridylyltransferase activity. However, the ATP ratio is higher than UTP in cells, suggesting that it functions primarily as a poly(A) polymerase. Acts as a specific terminal uridylyltransferase for U6 snRNA in vitro: responsible for a controlled elongation reaction that results in the restoration of the four 3'-terminal UMP-residues found in newly transcribed U6 snRNA. Not involved in replication-dependent histone mRNA degradation.
  • 组织特异性Widely expressed.
  • 序列相似性Belongs to the DNA polymerase type-B-like family.
    Contains 1 C2H2-type zinc finger.
    Contains 1 PAP-associated domain.
    Contains 1 RRM (RNA recognition motif) domain.
  • 翻译后修饰Phosphorylated by CK1 in the proline-rich (Pro-rich) region. Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位Nucleus > nucleolus. Nucleus speckle.
  • Information by UniProt
  • 数据库链接
  • 别名
    • alpha regulated poly(A) polymerase antibody
    • nuclear speckle targeted phosphatidylinositol 4 phosphate 5 kinase type I antibody
    • nuclear speckle targeted PIPK1A regulated poly(A) polymerase antibody
    • PAP associated domain containing 2 antibody
    • PAPD2 antibody
    • poly(A) polymerase associated domain containing 2 antibody
    • RBM21 antibody
    • RNA binding motif protein 21 antibody
    • RNA-binding motif protein 21 antibody
    • RNA-binding protein 21 antibody
    • Speckle targeted PIP5K1A-regulated poly(A) polymerase antibody
    • Star-PAP antibody
    • STARPAP antibody
    • STPAP_HUMAN antibody
    • terminal uridylyl transferase 1, U6 snRNA specific antibody
    • TUT1 antibody
    • TUTase antibody
    • U6 snRNA specific terminal uridylyltransferase 1 antibody
    • U6 snRNA-specific terminal uridylyltransferase 1 antibody
    • U6 TUTase antibody
    • U6-TUTase antibody
    see all

Anti-TUT1 antibody 图像

  • Anti-TUT1 antibody (ab86161) at 1 µg/ml (5% skim milk / PBS buffer) + 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 94 kDa
    Observed band size : 94 kDa
    Additional bands at : 45 kDa,70 kDa,>168 kDa. We are unsure as to the identity of these extra bands.

Anti-TUT1 antibody (ab86161)参考文献

ab86161 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab86161.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"