Anti-TUP1抗体[Clone 10] (ab24313)

概述

  • 产品名称Anti-TUP1抗体[Clone 10]
  • 描述
    小鼠单克隆抗体[Clone 10] to TUP1
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Saccharomyces cerevisiae
  • 免疫原

    Recombinant fragment, corresponding to amino acids 1-200 of Saccharomyces cerevisiae TUP1

  • 阳性对照
    • Mav108 cells expressing recombinant TUP1

性能

应用

Our Abpromise guarantee covers the use of ab24313 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 5 - 10 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 78 kDa).

靶标

  • 相关性Yeast TUP1 Ssn6p repressor complex represents a novel paradigm for transcriptional repression and for the role of chromatin in repression. TUP1 and SSN6 are involved in repression of several diverse families of genes in yeast, including cell type specific genes regulated by the alpha2 and a1/alpha2 repressors. Ssn6 Tupl interacts with class I histone deacetylases required for repression, and recruitment of yeast Tuplp Ssn6p repressor is associated with localised decreases in histone acetylation.
  • 细胞定位Nuclear
  • 数据库链接
    • 别名
      • FLK1 antibody
      • Flocculation suppressor protein antibody
      • Repressor AER2 antibody
      • SFL2 antibody
      • Tup1p antibody
      • UMR7 antibody
      see all

    Anti-TUP1 antibody [Clone 10] 图像

    • All lanes : Anti-TUP1 antibody [Clone 10] (ab24313)

      Lane 1 : Saccharomyces cerevisiae whole cell lysates
      Lane 2 : Saccharomyces cerevisiae whole cell lysates
      Lane 3 : Saccharomyces cerevisiae whole cell lysates

      Lysates/proteins at 15 µg per lane.

      Secondary
      Sheep anti-Mouse polyclonal HRP conjugate at 100 %
      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 78 kDa
      Observed band size : 90 kDa (why is the actual band size different from the predicted?)


      Exposure time : 1 minute

      Image is courtesy of an anonymous AbReview.

      See Abreview

    Anti-TUP1 antibody [Clone 10] (ab24313)参考文献

    ab24313 has not yet been referenced specifically in any publications.

    Product Wall

    Application Western blot
    Sample Saccharomyces cerevisiae Cell lysate - whole cell (whole cell)
    Gel Running Conditions Reduced Denaturing
    Loading amount 15 µg
    Specification whole cell
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

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    Verified customer

    提交于 Oct 30 2015

    Thank you for your enquiry. The cell line used was transfected to express a fragment of the TUP1 protein, which was the antigen used to develop the antibody. This protein fragment has a molecular weight of approximately 35 kDa. Therefore, the 35k...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"