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RabMAb

Anti-TUG抗体[EPR8616] (ab131217)

概述

  • 产品名称
    Anti-TUG抗体[EPR8616]
    参阅全部 TUG 一抗
  • 描述
    兔单克隆抗体[EPR8616] to TUG
  • 宿主
    Rabbit
  • 经测试应用
    适用于: WB, IPmore details
    不适用于: Flow Cyt,ICC or IHC-P
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to residues on the C-terminus of the Human TUG protein (Q9BZE9).

  • 阳性对照
    • K562, 293T, and SW480 cell lysates.
  • 常规说明

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

性能

应用

Our Abpromise guarantee covers the use of ab131217 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/10000 - 1/50000. Predicted molecular weight: 60 kDa.
IP 1/10 - 1/100.
  • 应用说明
    Is unsuitable for Flow Cyt,ICC or IHC-P.
  • 靶标

    • 功能
      Tethering protein that sequesters GLUT4-containing vesicles in the cytoplasm in the absence of insulin. Modulates the amount of GLUT4 that is available at the cell surface.
    • 组织特异性
      Ubiquitous. Highly expressed in testis, heart, skeletal muscle and pancreas.
    • 疾病相关
      Note=A chromosomal aberration involving ASPSCR1 is found in patients with alveolar soft part sarcoma. Translocation t(X;17)(p11;q25) with TFE3 forms a ASPSCR1-TFE3 fusion protein.
      Note=A chromosomal aberration involving ASPSCR1 has been found in two patients with of papillary renal cell carcinoma. Translocation t(X;17)(p11.2;q25).
    • 序列相似性
      Contains 1 UBX domain.
    • 细胞定位
      Endomembrane system.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Alveolar soft part sarcoma chromosomal region candidate gene 1 protein antibody
      • Alveolar soft part sarcoma chromosome region candidate 1 (human) antibody
      • Alveolar soft part sarcoma locus antibody
      • ASPC antibody
      • ASPC1_HUMAN antibody
      • ASPCR 1 antibody
      • ASPCR1 antibody
      • ASPL antibody
      • ASPS antibody
      • ASPSCR 1 antibody
      • Aspscr1 antibody
      • FLJ45380 antibody
      • RCC 17 antibody
      • RCC17 antibody
      • renal cell carcinoma gene on chromosome 17 antibody
      • renal cell carcinoma papillary 17 antibody
      • Renal papillary cell carcinoma protein 17 antibody
      • Tether containing a UBX domain for GLUT4 antibody
      • Tether containing UBX domain for GLUT4 antibody
      • TUG antibody
      • UBX domain containing protein 9 antibody
      • UBX domain protein 9 antibody
      • UBX domain-containing protein 9 antibody
      • UBXD 9 antibody
      • UBXD9 antibody
      • UBXN 9 antibody
      • UBXN9 antibody
      see all

    图片

    • Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: TUG knockout HAP1 cell lysate (20 µg)
      Lane 3: NIH/3T3 cell lysate (20 µg)
      Lane 4: HEK293 cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab131217 observed at 70 kDa. Red - loading control, ab8245, observed at 37 kDa.
      ab131217 was shown to specifically react with TUG when TUG knockout samples were used. Wild-type and TUG knockout samples were subjected to SDS-PAGE. ab131217 and ab8245 (loading control to GAPDH) were both diluted 1/10000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    • All lanes : Anti-TUG antibody [EPR8616] (ab131217) at 1/10000 dilution

      Lane 1 : K562 cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : SW480 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 60 kDa

    文献

    ab131217 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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