1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Is unsuitable for Flow Cyt or ICC.
Tethering protein that sequesters GLUT4-containing vesicles in the cytoplasm in the absence of insulin. Modulates the amount of GLUT4 that is available at the cell surface.
Ubiquitous. Highly expressed in testis, heart, skeletal muscle and pancreas.
Note=A chromosomal aberration involving ASPSCR1 is found in patients with alveolar soft part sarcoma. Translocation t(X;17)(p11;q25) with TFE3 forms a ASPSCR1-TFE3 fusion protein. Note=A chromosomal aberration involving ASPSCR1 has been found in two patients with of papillary renal cell carcinoma. Translocation t(X;17)(p11.2;q25).
Alveolar soft part sarcoma chromosomal region candidate gene 1 protein antibody
Alveolar soft part sarcoma chromosome region candidate 1 (human) antibody
Alveolar soft part sarcoma locus antibody
ASPCR 1 antibody
ASPSCR 1 antibody
RCC 17 antibody
renal cell carcinoma gene on chromosome 17 antibody
renal cell carcinoma papillary 17 antibody
Renal papillary cell carcinoma protein 17 antibody
Tether containing a UBX domain for GLUT4 antibody
Tether containing UBX domain for GLUT4 antibody
UBX domain containing protein 9 antibody
UBX domain protein 9 antibody
UBX domain-containing protein 9 antibody
UBXD 9 antibody
UBXN 9 antibody
Western blot - Anti-TUG antibody [EPR8615] (ab131237)
Lane 1: Wild-type HAP1 cell lysate (20 µg) Lane 2: TUG knockout HAP1 cell lysate (20 µg) Lane 3: NIH/3T3 cell lysate (20 µg) Lane 4: HEK293 cell lysate (20 µg) Lanes 1 - 4: Merged signal (red and green). Green - ab131237 observed at 70 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab131237 was shown to specifically react with TUG when TUG knockout samples were used. Wild-type and TUG knockout samples were subjected to SDS-PAGE. ab131237 and ab8245 (loading control to GAPDH) were both diluted 1/10 000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.