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RabMAb

Anti-TUG抗体[EPR8615] (ab131237)

概述

  • 产品名称
    Anti-TUG抗体[EPR8615]
    参阅全部 TUG 一抗
  • 描述
    兔单克隆抗体[EPR8615] to TUG
  • 宿主
    Rabbit
  • 经测试应用
    适用于: WB, IP, IHC-Pmore details
    不适用于: Flow Cyt or ICC
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to residues in human TUG (Q9BZE9).

  • 阳性对照
    • K562, 293T, and SW480 cell lysates for Western Blot and Human colon tissue for IHC-P.
  • 常规说明

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

性能

应用

Our Abpromise guarantee covers the use of ab131237 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/10000 - 1/50000. Predicted molecular weight: 60 kDa.
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • 应用说明
    Is unsuitable for Flow Cyt or ICC.
  • 靶标

    • 功能
      Tethering protein that sequesters GLUT4-containing vesicles in the cytoplasm in the absence of insulin. Modulates the amount of GLUT4 that is available at the cell surface.
    • 组织特异性
      Ubiquitous. Highly expressed in testis, heart, skeletal muscle and pancreas.
    • 疾病相关
      Note=A chromosomal aberration involving ASPSCR1 is found in patients with alveolar soft part sarcoma. Translocation t(X;17)(p11;q25) with TFE3 forms a ASPSCR1-TFE3 fusion protein.
      Note=A chromosomal aberration involving ASPSCR1 has been found in two patients with of papillary renal cell carcinoma. Translocation t(X;17)(p11.2;q25).
    • 序列相似性
      Contains 1 UBX domain.
    • 细胞定位
      Endomembrane system.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Alveolar soft part sarcoma chromosomal region candidate gene 1 protein antibody
      • Alveolar soft part sarcoma chromosome region candidate 1 (human) antibody
      • Alveolar soft part sarcoma locus antibody
      • ASPC antibody
      • ASPC1_HUMAN antibody
      • ASPCR 1 antibody
      • ASPCR1 antibody
      • ASPL antibody
      • ASPS antibody
      • ASPSCR 1 antibody
      • Aspscr1 antibody
      • FLJ45380 antibody
      • RCC 17 antibody
      • RCC17 antibody
      • renal cell carcinoma gene on chromosome 17 antibody
      • renal cell carcinoma papillary 17 antibody
      • Renal papillary cell carcinoma protein 17 antibody
      • Tether containing a UBX domain for GLUT4 antibody
      • Tether containing UBX domain for GLUT4 antibody
      • TUG antibody
      • UBX domain containing protein 9 antibody
      • UBX domain protein 9 antibody
      • UBX domain-containing protein 9 antibody
      • UBXD 9 antibody
      • UBXD9 antibody
      • UBXN 9 antibody
      • UBXN9 antibody
      see all

    图片

    • Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: TUG knockout HAP1 cell lysate (20 µg)
      Lane 3: NIH/3T3 cell lysate (20 µg)
      Lane 4: HEK293 cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab131237 observed at 70 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab131237 was shown to specifically react with TUG when TUG knockout samples were used. Wild-type and TUG knockout samples were subjected to SDS-PAGE. ab131237 and ab8245 (loading control to GAPDH) were both diluted 1/10 000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776  secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    • ab131237, at 1/100, staining TUG in Formalin-fixed, Paraffin-embedded Human colon tissue by Immunohistochemistry.
    • All lanes : Anti-TUG antibody [EPR8615] (ab131237) at 1/10000 dilution

      Lane 1 : K562 cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : SW480 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 60 kDa

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    文献

    ab131237 has not yet been referenced specifically in any publications.

    客户评价及客户问答

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Specification
    HeLa
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.05% Triton X100 in PBS
    Username

    Dr. Kirk Mcmanus

    Verified customer

    提交于 Apr 30 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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