The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.001 - 0.01 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 24 kDa).
Use a concentration of 3 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
May play a role in the polymerization of tubulin into microtubules, microtubule bundling and the stabilization of existing microtubules, thus maintaining the integrity of the microtubule network. May play a role in mitotic spindle assembly and nuclear envelope breakdown.
Belongs to the TPPP family.
Poor substrate for GSK3 (By similarity). Phosphorylated by LIMK1 on serine residues. Phosphorylation may alter the tubulin polymerization activity.
Cytoplasm. Cytoplasm, cytoskeleton. Nucleus. Localizes to glial Lewy bodies in the brains of individuals with synucleinopathies.
ab105033 (3.8µg/ml) staining of paraffin embedded Human Cerebral Cortex following Steamed antigen retrieval with citrate buffer pH 6 and AP-staining shows cytoskeletal-like staining throughout the fibre network.