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Our Abpromise guarantee covers the use of ab56676 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 5 µg/ml.|
|IHC-P||Use a concentration of 3 µg/ml.|
|Flow Cyt||Use 1-2µg for 106 cells.
ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
ab56676 at 1/500 dilution staining Tubulin in Human Osteosarcoma cells by immunocytochemistry/ Immunofluorescence. The cells were PFA fixed, permeabilized in PBS (0.5% Triton) and blocked in 1% BSA for 1 hour at 25°C. An Alexa Fluor® 488 conjugated Goat polyclonal to mouse IgG at 1/250 dilution, was used as secondary antibody. The primary antibody shows green staining in image.
This image is courtesy of an Abreview submitted by Xun AiBlocking Step : 5% BSA for 2 hours at 23°C