RabMAb

Anti-Tuberin (phospho T1462)抗体[EPR1060(2)] (ab109403)

概述

  • 产品名称Anti-Tuberin (phospho T1462)抗体[EPR1060(2)]
    参阅全部 Tuberin 一抗
  • 描述
    兔单克隆抗体[EPR1060(2)] to Tuberin (phospho T1462)
  • 特异性ab109403 only detects Tuberin phosphorylated at Threonine 1462.
  • 经测试应用适用于: WB, IHC-P, ICC/IFmore details
    不适用于: Flow Cyt or IP
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Phospho specific peptide against residues surrounding Threonine 1462 of Human Tuberin.

  • 阳性对照
    • NIH/3T3 cell lysate, Human liver tissue and HeLa cells.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

应用

Our Abpromise guarantee covers the use of ab109403 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Detects a band of approximately 180 kDa (predicted molecular weight: 201 kDa).
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.
  • 应用说明Is unsuitable for Flow Cyt or IP.
  • 靶标

    • 功能In complex with TSC1, inhibits the nutrient-mediated or growth factor-stimulated phosphorylation of S6K1 and EIF4EBP1 by negatively regulating mTORC1 signaling. Acts as a GTPase-activating protein (GAP) for the small GTPase RHEB, a direct activator of the protein kinase activity of mTORC1. Implicated as a tumor suppressor. Involved in microtubule-mediated protein transport, but this seems to be due to unregulated mTOR signaling. Stimulates weakly the intrinsic GTPase activity of the Ras-related proteins RAP1A and RAB5 in vitro. Mutations in TSC2 lead to constitutive activation of RAP1A in tumors.
    • 组织特异性Liver, brain, heart, lymphocytes, fibroblasts, biliary epithelium, pancreas, skeletal muscle, kidney, lung and placenta.
    • 疾病相关Defects in TSC2 are the cause of tuberous sclerosis type 2 (TSC2) [MIM:613254]. TSC2 is an autosomal dominant multi-system disorder that affects especially the brain, kidneys, heart, and skin. It is characterized by hamartomas (benign overgrowths predominantly of a cell or tissue type that occurs normally in the organ) and hamartias (developmental abnormalities of tissue combination). Clinical symptoms can range from benign hypopigmented macules of the skin to profound mental retardation with intractable seizures to premature death from a variety of disease-associated causes.
      Defects in TSC2 are a cause of lymphangioleiomyomatosis (LAM) [MIM:606690]. LAM is a progressive and often fatal lung disease characterized by a diffuse proliferation of abnormal smooth muscle cells in the lungs. It affects almost exclusively young women and can occur as an isolated disorder or in association with tuberous sclerosis complex.
    • 序列相似性Contains 1 Rap-GAP domain.
    • 翻译后修饰Phosphorylation at Ser-1387, Ser-1418 or Ser-1420 does not affect interaction with TSC1.
      Phosphorylation at Ser-939 and Thr-1462 by PKB/AKT1 is induced by growth factor stimulation.
    • 细胞定位Cytoplasm. Membrane. At steady state found in association with membranes.
    • Information by UniProt
    • 数据库链接
    • 别名
      • FLJ43106 antibody
      • LAM antibody
      • OTTHUMP00000158940 antibody
      • OTTHUMP00000198394 antibody
      • OTTHUMP00000198395 antibody
      • PPP1R160 antibody
      • Protein phosphatase 1, regulatory subunit 160 antibody
      • tsc2 antibody
      • TSC2_HUMAN antibody
      • TSC4 antibody
      • TSC4 gene, formerly antibody
      • TSC4, formerly antibody
      • Tuberin antibody
      • Tuberous sclerosis 2 antibody
      • Tuberous sclerosis 2 protein antibody
      • Tuberous sclerosis 2 protein homolog antibody
      see all

    Anti-Tuberin (phospho T1462) antibody [EPR1060(2)] 图像

    • All lanes : Anti-Tuberin (phospho T1462) antibody [EPR1060(2)] (ab109403) at 1/1000 dilution

      Lane 1 : NIH/3T3 cell lysate
      Lane 2 : NIH/3T3 cell lysate treated with insulin.

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 201 kDa
      Observed band size : 180 kDa (why is the actual band size different from the predicted?)
    • Immunofluorescent staining of Tuberin in HeLa cells using ab109403 at 1/100.
    • Immunohistochemical analysis of paraffin-embedded Human liver tissue using ab109403 at 1/50

    Anti-Tuberin (phospho T1462) antibody [EPR1060(2)] (ab109403)参考文献

    ab109403 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab109403.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"