Anti-Tuberin (phospho T1462)抗体(ab59274)

概述

  • 产品名称
    Anti-Tuberin (phospho T1462)抗体
    参阅全部 Tuberin 一抗
  • 描述
    兔多克隆抗体to Tuberin (phospho T1462)
  • 特异性
    ab59274 detects endogenous levels of Tuberin only when phosphorylated at threonine 1462.
  • 经测试应用
    适用于: IHC-P, ELISAmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    A synthesized phosphopeptide derived from human Tuberin around the phosphorylation site of threonine 1462 (G-Y-TP-I-S).

  • 阳性对照
    • Human breast carcinoma tissue.

性能

应用

Our Abpromise guarantee covers the use of ab59274 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent concentration.
ELISA 1/5000.

靶标

  • 功能
    In complex with TSC1, inhibits the nutrient-mediated or growth factor-stimulated phosphorylation of S6K1 and EIF4EBP1 by negatively regulating mTORC1 signaling. Acts as a GTPase-activating protein (GAP) for the small GTPase RHEB, a direct activator of the protein kinase activity of mTORC1. Implicated as a tumor suppressor. Involved in microtubule-mediated protein transport, but this seems to be due to unregulated mTOR signaling. Stimulates weakly the intrinsic GTPase activity of the Ras-related proteins RAP1A and RAB5 in vitro. Mutations in TSC2 lead to constitutive activation of RAP1A in tumors.
  • 组织特异性
    Liver, brain, heart, lymphocytes, fibroblasts, biliary epithelium, pancreas, skeletal muscle, kidney, lung and placenta.
  • 疾病相关
    Defects in TSC2 are the cause of tuberous sclerosis type 2 (TSC2) [MIM:613254]. TSC2 is an autosomal dominant multi-system disorder that affects especially the brain, kidneys, heart, and skin. It is characterized by hamartomas (benign overgrowths predominantly of a cell or tissue type that occurs normally in the organ) and hamartias (developmental abnormalities of tissue combination). Clinical symptoms can range from benign hypopigmented macules of the skin to profound mental retardation with intractable seizures to premature death from a variety of disease-associated causes.
    Defects in TSC2 are a cause of lymphangioleiomyomatosis (LAM) [MIM:606690]. LAM is a progressive and often fatal lung disease characterized by a diffuse proliferation of abnormal smooth muscle cells in the lungs. It affects almost exclusively young women and can occur as an isolated disorder or in association with tuberous sclerosis complex.
  • 序列相似性
    Contains 1 Rap-GAP domain.
  • 翻译后修饰
    Phosphorylation at Ser-1387, Ser-1418 or Ser-1420 does not affect interaction with TSC1.
    Phosphorylation at Ser-939 and Thr-1462 by PKB/AKT1 is induced by growth factor stimulation.
  • 细胞定位
    Cytoplasm. Membrane. At steady state found in association with membranes.
  • Information by UniProt
  • 数据库链接
  • 别名
    • FLJ43106 antibody
    • LAM antibody
    • OTTHUMP00000158940 antibody
    • OTTHUMP00000198394 antibody
    • OTTHUMP00000198395 antibody
    • PPP1R160 antibody
    • Protein phosphatase 1, regulatory subunit 160 antibody
    • TSC complex subunit 2 antibody
    • tsc2 antibody
    • TSC2_HUMAN antibody
    • TSC4 antibody
    • TSC4 gene, formerly antibody
    • TSC4, formerly antibody
    • Tuberin antibody
    • Tuberous sclerosis 2 antibody
    • Tuberous sclerosis 2 protein antibody
    • Tuberous sclerosis 2 protein homolog antibody
    see all

图片

  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ab59274 at a dilution of 1/50, in the presence (right) or absence (left) of immunising peptide.

文献

This product has been referenced in:
  • Presneau N  et al. Potential therapeutic targets for chordoma: PI3K/AKT/TSC1/TSC2/mTOR pathway. Br J Cancer 100:1406-14 (2009). WB, ICC/IF, IHC-P ; Human . Read more (PubMed: 19401700) »

See 1 Publication for this product

客户评价及客户问答

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (multi-cancer block (breast, colon, lung, thyroid,)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EDTA, pH9, 100C, 20min
Permeabilization
No
Specification
multi-cancer block (breast, colon, lung, thyroid,
Blocking step
H2O2 as blocking agent for 10 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative
Formaldehyde
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提交于 May 05 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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