概述

  • 产品名称Anti-Tubby抗体
    参阅全部 Tubby 一抗
  • 描述
    兔多克隆抗体to Tubby
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Rabbit, Horse
  • 免疫原

    Synthetic peptide, corresponding to a region within N terminal amino acids 0-49 (MGARTPLPSFWVSFFAETGILFPGGTPWPMGSQHSKQHRKPGPLKRGHR R) of Human Tubby (NP_003311)

  • 阳性对照
    • 293T cell lysate.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明ab82689 is purified by a peptide affinity chromatography method.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab82689 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 62 kDa (predicted molecular weight: 62 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/1562500.

靶标

  • 相关性Tubby is a bipartite transcription factor. It may play a role in obesity and sensorineural degradation. The crystal structure has been determined for a similar protein in mouse, which functions as a membrane bound transcription regulator that translocates to the nucleus in response to phosphoinositide hydrolysis.
  • 细胞定位Cytoplasm. Nucleus. Secreted. Cell membrane. Binds phospholipid and is anchored to the plasma membrane through binding phosphatidylinositol 4,5-bisphosphate. Is released upon activation of phospholipase C. Translocates from the plasma membrane to the nucleus upon activation of guanine nucleotide-binding protein G(q) subunit alpha. Does not have a cleavable signal peptide and is secreted by a non-conventional pathway.
  • 数据库链接
  • 别名
    • Mouse tubby homologue antibody
    • rd5 antibody
    • Retinal degeneration 5 antibody
    • TUB antibody
    • Tubby (mouse) homolog antibody
    • tubby antibody
    • Tubby homolog (mouse) antibody
    • Tubby homologue antibody
    • Tubby protein homolog antibody
    see all

Anti-Tubby antibody 图像

  • Anti-Tubby antibody (ab82689) at 1 µg/ml + 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 62 kDa
    Observed band size : 62 kDa
    Additional bands at : 50 kDa. We are unsure as to the identity of these extra bands.

Anti-Tubby antibody (ab82689)参考文献

ab82689 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"