Anti-Tubby抗体(ab46943)

概述

  • 产品名称Anti-Tubby抗体
    参阅全部 Tubby 一抗
  • 描述
    兔多克隆抗体to Tubby
  • 经测试应用适用于: IHC-P, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Mouse Tubby.

    (Peptide available as ab47529.)

  • 阳性对照
    • This antibody gave a positive signal in the following tissue lysates: Human Spinal Cord, Mouse Brain, Mouse Testis, Mouse Spinal Cord, Rat Brain and Rat Dorsal Root Ganglion.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab46943 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).

靶标

  • 相关性Tubby is a bipartite transcription factor. It may play a role in obesity and sensorineural degradation. The crystal structure has been determined for a similar protein in mouse, which functions as a membrane bound transcription regulator that translocates to the nucleus in response to phosphoinositide hydrolysis.
  • 细胞定位Cytoplasm. Nucleus. Secreted. Cell membrane. Binds phospholipid and is anchored to the plasma membrane through binding phosphatidylinositol 4,5-bisphosphate. Is released upon activation of phospholipase C. Translocates from the plasma membrane to the nucleus upon activation of guanine nucleotide-binding protein G(q) subunit alpha. Does not have a cleavable signal peptide and is secreted by a non-conventional pathway.
  • 数据库链接
  • 别名
    • Mouse tubby homologue antibody
    • rd5 antibody
    • Retinal degeneration 5 antibody
    • TUB antibody
    • Tubby (mouse) homolog antibody
    • tubby antibody
    • Tubby homolog (mouse) antibody
    • Tubby homologue antibody
    • Tubby protein homolog antibody
    see all

Anti-Tubby antibody 图像

  • All lanes : Anti-Tubby antibody (ab46943) at 1 µg/ml

    Lane 1 : Human spinal cord tissue lysate - total protein (ab29188)
    Lane 2 : Brain (Mouse) Tissue Lysate
    Lane 3 : Testis (Mouse) Tissue Lysate - normal tissue
    Lane 4 : Spinal Cord (Mouse) Tissue Lysate
    Lane 5 : Brain (Rat) Tissue Lysate - normal tissue
    Lane 6 : Rat Dorsal Root Ganglion

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 55 kDa
    Observed band size : 55 kDa
  • IHC image of Tubby staining in human cerebral cortex formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab46943, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Anti-Tubby antibody (ab46943)参考文献

ab46943 has not yet been referenced specifically in any publications.

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They confirmed the mouse strain used is CD-1 and the animals used are surplus animals over 8 weeks old.

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The lysates were prepared using RIPA buffer and phosphatase inhibitors. The gel was run under reducing conditions, and the blocking was pe...

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