概述

  • 产品名称Anti-TUBB8抗体
    参阅全部 TUBB8 一抗
  • 描述
    兔多克隆抗体to TUBB8
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Rat, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to a region within amino acids 366 and 430 of Human TUBB8 (NP_817124).

  • 阳性对照
    • 293T, A431, H1299, HeLaS3, HepG2, MOLT4 and Raji cell lines

性能

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应用

Our Abpromise guarantee covers the use of ab97880 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/3000. Predicted molecular weight: 50 kDa.
ICC/IF 1/100 - 1/200.

靶标

  • 功能Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
  • 序列相似性Belongs to the tubulin family.
  • 翻译后修饰Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
  • 细胞定位Cytoplasm > cytoskeleton.
  • Information by UniProt
  • 数据库链接
  • 别名
    • bA631M21.2 antibody
    • Class VIII beta tubulin antibody
    • HSA10p15 beta tubulin 4Q antibody
    • RP11-631M21.2 antibody
    • TBB8_HUMAN antibody
    • TUBB8 antibody
    • Tubulin beta 8 antibody
    • Tubulin beta 8 chain antibody
    • Tubulin beta 8 class VIII antibody
    • Tubulin beta-8 chain antibody
    see all

Anti-TUBB8 antibody 图像

  • All lanes : Anti-TUBB8 antibody (ab97880) at 1/1000 dilution

    Lane 1 : A431 whole cell lysate
    Lane 2 : H1299 whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 50 kDa
  • Immunofluorescence analysis of TUBB8 in paraformaldehyde fixed HeLa, using ab97880 at a 1/200 dilution.
    Lower image: merged with DNA probe.

Anti-TUBB8 antibody (ab97880)参考文献

ab97880 has not yet been referenced specifically in any publications.

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