概述

  • 产品名称Anti-TUBB2A抗体
    参阅全部 TUBB2A 一抗
  • 描述
    小鼠单克隆抗体to TUBB2A
  • 经测试应用适用于: WB, ELISA, IHC-P, ICC/IF, Flow Cytmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Recombinant full length protein, corresponding to amino acids 1 - 446 of Human TUBB2A (AAH01194), with a proprietary tag .

  • 阳性对照
    • WB: PC12, Jurkat or NIH 3T3 cell lysate; IHC-P: Human colon tissue. IF/ICC: SW480 cell line.

性能

应用

Our Abpromise guarantee covers the use of ab92857 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 50 kDa.
ELISA Use at an assay dependent concentration.
IHC-P Use a concentration of 6 µg/ml. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
ICC/IF Use a concentration of 5 µg/ml.
Flow Cyt Use 0.01µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

靶标

  • 功能Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.
  • 序列相似性Belongs to the tubulin family.
  • 翻译后修饰Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
  • 细胞定位Cytoplasm > cytoskeleton.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Class IIa beta tubulin antibody
    • dJ40E16.7 antibody
    • M(beta)2 antibody
    • TBB2A_HUMAN antibody
    • TUBB antibody
    • TUBB2 antibody
    • Tubb2a antibody
    • Tubulin beta 2 antibody
    • Tubulin beta 2A antibody
    • Tubulin beta 2A class IIa antibody
    • Tubulin beta polypeptide 2 antibody
    • Tubulin beta polypeptide antibody
    • Tubulin beta-2A chain antibody
    see all

Anti-TUBB2A antibody 图像

  • ab92857 stained SW480 cells. The cells were 100% methanol fixed for 5 minutes at -20°C and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab92857 at 5µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed (ab150117) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

  • Overlay histogram showing Jurkat cells stained with ab92857 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab92857, 0.01μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in Jurkat cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
  • Anti-TUBB2A antibody (ab92857) at 5 µg/ml + PC12 cell lysate at 50 µg

    Secondary
    HRP conjugated Goat anti-Mouse IgG at 1/2500 dilution

    Predicted band size : 50 kDa
  • Anti-TUBB2A antibody (ab92857) at 5 µg/ml + NIH 3T3 cell lysate at 50 µg

    Secondary
    HRP conjugated Goat anti-Mouse IgG at 1/2500 dilution

    Predicted band size : 50 kDa
  • Anti-TUBB2A antibody (ab92857) at 5 µg/ml + Jurkat cell lysate at 50 µg

    Secondary
    HRP conjugated Goat anti-Mouse IgG at 1/2500 dilution

    Predicted band size : 50 kDa
  • ab92857 at 6µg/ml staining TUBB2A in formalin-fixed, paraffin-embedded Human colon tissue.

Anti-TUBB2A antibody (ab92857)参考文献

This product has been referenced in:
  • Kaldma A  et al. An in situ study of bioenergetic properties of human colorectal cancer: The regulation of mitochondrial respiration and distribution of flux control among the components of ATP synthasome. Int J Biochem Cell Biol 55C:171-186 (2014). Read more (PubMed: 25218857) »

See 1 Publication for this product

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