Anti-TUBA4A (acetyl K40)抗体[6-11B-1] (ab11323)

概述

  • 产品名称Anti-TUBA4A (acetyl K40)抗体[6-11B-1]
    参阅全部 TUBA4A 一抗
  • 描述
    小鼠单克隆抗体[6-11B-1] to TUBA4A (acetyl K40)
  • 特异性ab11323 recognizes an epitope located on the a3 isoform of Chlamydomonas axonemal a-tubulin, within four residues of Lys-40 when this amino acid is acetylated. A sequence very similar to the one detected by ab11323 in Chlamydomonas is found in the majority of a-tubulins, but the corresponding region is markedly divergent in some a-tubulin isoforms from chicken, Drosophila and yeast. The antibody has been used to detect acetylated a-tubulins in several organisms. Occasionally, the epitope recognized by the antibody may be absent or masked as it is in the kangaroo rat epithelial-like cell line PtK2.
  • 经测试应用适用于: WB, ELISA, Dot blot, RIA, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Chicken, Hamster, Cow, Dog, Human, Pig, Xenopus laevis, Caenorhabditis elegans, Chlamydomonas reinhardtii, Sea Urchin
  • 免疫原

    Acetylated TUBA4A from the outer arm of sea urchin sperm axonemes.

  • 常规说明Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

性能

应用

Our Abpromise guarantee covers the use of ab11323 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000. This dilution was determined using bovine or rat brain preparation.
ELISA Use at an assay dependent concentration.
Dot blot Use at an assay dependent concentration.
RIA Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. PubMed: 23269434
ICC/IF Use at an assay dependent concentration.

靶标

  • 功能Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.
  • 序列相似性Belongs to the tubulin family.
  • 翻译后修饰Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
    Acetylation of alpha-tubulins at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling.
  • 细胞定位Cytoplasm > cytoskeleton.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Alpha tubulin 1 antibody
    • Alpha-tubulin 1 antibody
    • FLJ30169 antibody
    • H2 alpha antibody
    • TBA4A_HUMAN antibody
    • Testis specific alpha tubulin antibody
    • Testis-specific alpha-tubulin antibody
    • TUBA 4A antibody
    • TUBA1 antibody
    • Tuba4a antibody
    • Tubulin alpha 1 (testis specific) antibody
    • Tubulin alpha 1 antibody
    • Tubulin alpha 1 chain antibody
    • Tubulin alpha 4a antibody
    • Tubulin alpha 4A chain antibody
    • Tubulin alpha-1 chain antibody
    • Tubulin alpha-4A chain antibody
    • Tubulin H2 alpha antibody
    • Tubulin H2-alpha antibody
    see all

Anti-TUBA4A (acetyl K40) antibody [6-11B-1] 图像

  • ab11323 at 1/800 staining dog kidney cells by ICC/IF. The cells were paraformaldehyde fixed and blocked with BSA prior to incubation with the antibody for 1 hour. An Alexa-Fluor ® 488 conjugated goat anti-mouse IgG was used as the secondary.

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  • All lanes : Anti-TUBA4A (acetyl K40) antibody [6-11B-1] (ab11323) at 1/1000 dilution

    Lane 1 : Whole animal lysate prepared from wild type (N2) C. Elegans worms.
    Lane 2 : Whole animal lysate prepared from elpc-1 C. Elegans worms.
    Lane 3 : Whole animal lysate prepared from elpc-3 C. Elegans worms.
    Lane 4 : Whole animal lysate prepared from mec-12 (e1605) C. Elegans worms.
    Lane 5 : Whole animal lysate prepared from mec-12 (e1607) C. Elegans worms.

    Lysates/proteins at 20 µg per lane.

    Image from Chen C et al, PLoS Genet. 2009 Jul;5(7):e1000561. Epub 2009 Jul 10, fig S9.

  • Anti-TUBA4A (acetyl K40) antibody [6-11B-1] (ab11323) at 1/2000 dilution (in PBST + 2.5% milk for 16 hours at 4°C) + Whole cell lysate of human IHP1 cells at 30 µg

    Secondary
    An HRP-conjugated Goat anti-mouse IgG monoclonal at 1/2000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Observed band size : 55 kDa (why is the actual band size different from the predicted?)


    Exposure time : 20 seconds

    This image is courtesy of an anonymous Abreview

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  • ab11323 staining Tubulin (acetyl K40) in human glioblastoma tumor sphere cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Triton X-100 0.1% in PBS and blocked with 0.5% BSA for 20 minutes at room temperature. Samples were incubated with primary antibody (1/100 in PBS + 0.5% BSA) for 1 hour at 4°C. An Alexa Fluor®488-conjugated Goat anti-mouse IgG polyclonal (1/400) was used as the secondary antibody. Nuclei were counterstained with DAPI (blue).

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Anti-TUBA4A (acetyl K40) antibody [6-11B-1] (ab11323)参考文献

This product has been referenced in:
  • Caro-Astorga J  et al. Nascent histamine induces a-synuclein and caspase-3 on human cells. Biochem Biophys Res Commun 451:580-6 (2014). WB . Read more (PubMed: 25124665) »
  • Li Z  et al. Coxsackievirus A16 infection induces neural cell and non-neural cell apoptosis in vitro. PLoS One 9:e111174 (2014). Read more (PubMed: 25350381) »

See all 5 Publications for this product

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I am now able to confirm that these antibodies are produced from the same clone 6-11B-1 and should produce very similar results. We have them separated into two diffe...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Cell lysate - whole cell (hepatocytes)
Loading amount 15 µg
Specification hepatocytes
Gel Running Conditions Reduced Denaturing (12%)
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5%
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Verified customer

提交于 Jun 01 2012

Thank you for calling Abcam earlier today.

As we discussed on the telephone earlier, I have sent youan aliquot of ab3954, PC3 (Human prostate cancer cell line) Whole Cell Lysate to test ab11323 with.

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (human glioblastoma tumor sphere cells)
Specification human glioblastoma tumor sphere cells
Fixative Paraformaldehyde
Permeabilization Yes - Tritonx100 0.1% in PBS
Blocking step BSA as blocking agent for 20 minute(s) · Concentration: 0.5% · Temperature: RT°C
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Mr. Lars Rüther

Verified customer

提交于 Nov 22 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (3T3-L1)
Loading amount 30 µg
Specification 3T3-L1
Gel Running Conditions Reduced Denaturing (12%)
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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提交于 Jul 08 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (THP-1 cells)
Loading amount 30 µg
Specification THP-1 cells
Gel Running Conditions Reduced Denaturing (12%)
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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Verified customer

提交于 Jul 05 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Dog Cell (kidney)
Specification kidney
Fixative Methanol
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1%
Username

Abcam user community

Verified customer

提交于 Jun 21 2007

Thank you for your email. I would suggest beginning with dilutions of 1:50, 1:100, and 1:200 and adjust dilutions appropriately from the results obtained. If you have any more questions, please contact us again.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"