概述

  • 产品名称Anti-TUBA3C抗体
    参阅全部 TUBA3C 一抗
  • 描述
    兔多克隆抗体to TUBA3C
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Sheep, Rabbit, Dog, Pig, Caenorhabditis elegans, Drosophila melanogaster, Zebrafish
  • 免疫原

    Synthetic peptide from Human TUBA3C

  • 阳性对照
    • HepG2 cell lysate.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab75069 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 50 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent dilution.

靶标

  • 功能Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.
  • 组织特异性Testis specific.
  • 序列相似性Belongs to the tubulin family.
  • 翻译后修饰Undergoes a tyrosination/detyrosination cycle, the cyclic removal and re-addition of a C-terminal tyrosine residue by the enzymes tubulin tyrosine carboxypeptidase (TTCP) and tubulin tyrosine ligase (TTL), respectively.
    Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
    Acetylation of alpha-tubulins at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling.
  • 细胞定位Cytoplasm > cytoskeleton.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Alpha-tubulin 2 antibody
    • Alpha-tubulin 3C/D antibody
    • alpha-tubulin isotype H2-alpha antibody
    • H2-ALPHA antibody
    • TBA3C_HUMAN antibody
    • TUBA2 antibody
    • TUBA3C antibody
    • TUBA3D antibody
    • Tubulin alpha 2 antibody
    • Tubulin alpha 2 chain antibody
    • Tubulin alpha 3c antibody
    • Tubulin alpha-2 chain antibody
    • Tubulin alpha-3C/D chain antibody
    see all

Anti-TUBA3C antibody 图像



  • Predicted band size : 50 kDa
    Western blot analysis of Jurkat cell lysate labeling TUBA3C with ab75069 at 1.0µg/ml.


  • Predicted band size : 50 kDa
    Western blot analysis of HeLa cell lysate labeling TUBA3C with ab75069 at 1.0µg/ml.
  • Anti-TUBA3C antibody (ab75069) at 1 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 50 kDa
    Observed band size : 50 kDa

Anti-TUBA3C antibody (ab75069)参考文献

ab75069 has not yet been referenced specifically in any publications.

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