概述

  • 产品名称
  • 描述
    兔多克隆抗体to TUBA3C
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide conjugated to KLH, corresponding to a region within C terminal amino acids 412-441 of Human TUBA3C (NP_525125.2, NP_005992.1).

  • 阳性对照
    • MDA-MB435 cell line lysates.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
  • 存储溶液
    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    ab116219 is purified through a protein A column, followed by peptide affinity purification.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab116219 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/100 - 1/500. Predicted molecular weight: 50 kDa.

靶标

  • 功能
    Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.
  • 组织特异性
    Testis specific.
  • 序列相似性
    Belongs to the tubulin family.
  • 翻译后修饰
    Undergoes a tyrosination/detyrosination cycle, the cyclic removal and re-addition of a C-terminal tyrosine residue by the enzymes tubulin tyrosine carboxypeptidase (TTCP) and tubulin tyrosine ligase (TTL), respectively.
    Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
    Acetylation of alpha-tubulins at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling.
  • 细胞定位
    Cytoplasm > cytoskeleton.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Alpha-tubulin 2 antibody
    • Alpha-tubulin 3C/D antibody
    • alpha-tubulin isotype H2-alpha antibody
    • H2-ALPHA antibody
    • TBA3C_HUMAN antibody
    • TUBA2 antibody
    • TUBA3C antibody
    • TUBA3D antibody
    • Tubulin alpha 2 antibody
    • Tubulin alpha 2 chain antibody
    • Tubulin alpha 3c antibody
    • Tubulin alpha-2 chain antibody
    • Tubulin alpha-3C/D chain antibody
    see all

图片

  • Anti-TUBA3C antibody (ab116219) at 1/100 dilution + MDA-MB435 cell line lysate at 35 µg

    Predicted band size : 50 kDa

实验方案

文献

ab116219 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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