概述

  • 产品名称Anti-TUBA1B抗体[EPR1333]
    参阅全部 TUBA1B 一抗
  • 描述
    兔单克隆抗体[EPR1333] to TUBA1B
  • 经测试应用适用于: WB, IHC-P, Flow Cyt, ICC/IFmore details
    不适用于: IP
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    A synthetic peptide corresponding to residues in Human TUBA1B.

  • 阳性对照
    • WB: HeLa, Jurkat, 293T, and A431 cell lysates IHC-P: Human placenta and breast tissues ICC/IF: HeLa cells
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

性能

应用

Our Abpromise guarantee covers the use of ab108629 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/10000 - 1/50000. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).
IHC-P 1/500 - 1/1000. Antigen retrieval is recommended.
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF 1/250 - 1/500.
  • 应用说明Is unsuitable for IP.
  • 靶标

    • 功能Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.
    • 序列相似性Belongs to the tubulin family.
    • 翻译后修饰Undergoes a tyrosination/detyrosination cycle, the cyclic removal and re-addition of a C-terminal tyrosine residue by the enzymes tubulin tyrosine carboxypeptidase (TTCP) and tubulin tyrosine ligase (TTL), respectively.
      Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
      Acetylation of alpha-tubulins at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling.
    • 细胞定位Cytoplasm > cytoskeleton.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Alpha tubulin ubiquitous antibody
      • Alpha-tubulin ubiquitous antibody
      • K alpha 1 antibody
      • TBA1B_HUMAN antibody
      • TUBA1B antibody
      • Tubulin alpha 1B antibody
      • Tubulin alpha 1B chain antibody
      • Tubulin alpha ubiquitous antibody
      • Tubulin alpha ubiquitous chain antibody
      • Tubulin alpha-1B chain antibody
      • Tubulin alpha-ubiquitous chain antibody
      • Tubulin K alpha 1 antibody
      • Tubulin K-alpha-1 antibody
      see all

    Anti-TUBA1B antibody [EPR1333] 图像

    • Immunocytochemistry/Immunofluorescence analysis of Jurkat (human acute T cell leukemia) labelling TUBA1B with purified ab108629 at 1/500. Cells were fixed with 100% methanol. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody (Ab150077). Nuclei counterstained with DAPI (blue).

      Control: PBS only

    • All lanes : Anti-TUBA1B antibody [EPR1333] (ab108629) at 1/10000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : 293T cell lysate
      Lane 4 : A431 cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 50 kDa
      Observed band size : 50 kDa
    • ab108629, at 1/500 dilution, staining TUBA1B in paraffin embedded Human placenta tissue by Immunohistochemistry.
    • ab108629, at a 1/500 dilution, staining TUBA1B in paraffin embedded Human breast tissue by Immunohistochemistry.
    • ab108629, at a 1/250 dilution, staining TUBA1B in HeLa cells by Immunofluorescence.

    Anti-TUBA1B antibody [EPR1333] (ab108629)参考文献

    This product has been referenced in:
    • Zheng YC  et al. 1,2,3-Triazole-Dithiocarbamate Hybrids, a Group of Novel Cell Active SIRT1 Inhibitors. Cell Physiol Biochem 38:185-93 (2016). WB ; Human . Read more (PubMed: 26784898) »
    • Mei Z  et al. FBXO32 Targets c-Myc for Proteasomal Degradation and Inhibits c-Myc Activity. J Biol Chem 290:16202-14 (2015). Human . Read more (PubMed: 25944903) »

    See all 5 Publications for this product

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