概述

  • 产品名称Anti-TUBA1B抗体
    参阅全部 TUBA1B 一抗
  • 描述
    兔多克隆抗体to TUBA1B
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Cow, Zebrafish
  • 免疫原

    Recombinant fragment, corresponding to a region within amino acids 118-375 of Human TUBA1B (NP_006073)

  • 阳性对照
    • HeLa, A431, 293T, H1299 and Raji cells.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 10% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab97622 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/3000. Predicted molecular weight: 50 kDa.
ICC/IF 1/100 - 1/200.

靶标

  • 功能Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.
  • 序列相似性Belongs to the tubulin family.
  • 翻译后修饰Undergoes a tyrosination/detyrosination cycle, the cyclic removal and re-addition of a C-terminal tyrosine residue by the enzymes tubulin tyrosine carboxypeptidase (TTCP) and tubulin tyrosine ligase (TTL), respectively.
    Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
    Acetylation of alpha-tubulins at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling.
  • 细胞定位Cytoplasm > cytoskeleton.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Alpha tubulin ubiquitous antibody
    • Alpha-tubulin ubiquitous antibody
    • K alpha 1 antibody
    • TBA1B_HUMAN antibody
    • TUBA1B antibody
    • Tubulin alpha 1B antibody
    • Tubulin alpha 1B chain antibody
    • Tubulin alpha ubiquitous antibody
    • Tubulin alpha ubiquitous chain antibody
    • Tubulin alpha-1B chain antibody
    • Tubulin alpha-ubiquitous chain antibody
    • Tubulin K alpha 1 antibody
    • Tubulin K-alpha-1 antibody
    see all

Anti-TUBA1B antibody 图像

  • Anti-TUBA1B antibody (ab97622) at 1/1000 dilution + HeLa whole cell lysate at 30 µg

    Predicted band size : 50 kDa
  • Immunofluorescence analysis of paraformaldehyde-fixed A431, using ab97622 at 1/200 dilution. Bottom picture shows stain merged with DNA probe.

Anti-TUBA1B antibody (ab97622)参考文献

ab97622 has not yet been referenced specifically in any publications.

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