The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用说明IHC-P: Use neat for 30 minutes at room temperature.
Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10mM citrate buffer, pH 6.0 prior to immunostaining.
IHC-Fr (acetone-fixed): Use neat for 30 minutes at room temperature.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
功能Transcription factor that binds and activates the promoter of thyroid specific genes such as thyroglobulin, thyroperoxidase, and thyrotropin receptor. Crucial in the maintenance of the thyroid differentiation phenotype. May play a role in lung development and surfactant homeostasis.
组织特异性Thyroid and lung.
疾病相关Defects in NKX2-1 are the cause of benign hereditary chorea (BHC) [MIM:118700]; also known as hereditary chorea without dementia. BHC is an autosomal dominant movement disorder. The early onset of symptoms (usully before the age of 5) and the observation that in some BHC families the symptoms tend to decrease in adulthood suggests that the disorder results from a developmental disturbance of the brain. BHC is non-progressive and patients have normal or slightly below normal intelligence. There is considerable inter- and intrafamilial variability, including dysarthria, axial distonia and gait disturbances. Defects in NKX2-1 are the cause of choreoathetosis, hypothyroidism, and neonatal respiratory distress (CHNRD) [MIM:610978]. This syndrome include neurological, thyroid, and respiratory problems.
序列相似性Belongs to the NK-2 homeobox family. Contains 1 homeobox DNA-binding domain.