Anti-TTF1抗体[8G7G3/1] (ab72876)


  • 产品名称Anti-TTF1抗体[8G7G3/1]
    参阅全部 TTF1 一抗
  • 描述
    小鼠单克隆抗体[8G7G3/1] to TTF1
  • 经测试应用适用于: IHC-P, IHC-Fr, Flow Cytmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Recombinant full length rat TTF1 protein

  • 阳性对照
    • Normal thyroid or lung tissue.


  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.1% Sodium Azide
    Constituents: 1% BSA, 10mM PBS, pH 7.4
  • Concentration information loading...
  • 纯度IgG fraction
  • 克隆单克隆
  • 克隆编号8G7G3/1
  • 同种型IgG1
  • 轻链类型kappa
  • 研究领域


Our Abpromise guarantee covers the use of ab72876 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/50. PMID 20081193
IHC-Fr 1/50.
Flow Cyt Use 20µl for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.


  • 功能Transcription factor that binds and activates the promoter of thyroid specific genes such as thyroglobulin, thyroperoxidase, and thyrotropin receptor. Crucial in the maintenance of the thyroid differentiation phenotype. May play a role in lung development and surfactant homeostasis.
  • 组织特异性Thyroid and lung.
  • 疾病相关Defects in NKX2-1 are the cause of benign hereditary chorea (BHC) [MIM:118700]; also known as hereditary chorea without dementia. BHC is an autosomal dominant movement disorder. The early onset of symptoms (usully before the age of 5) and the observation that in some BHC families the symptoms tend to decrease in adulthood suggests that the disorder results from a developmental disturbance of the brain. BHC is non-progressive and patients have normal or slightly below normal intelligence. There is considerable inter- and intrafamilial variability, including dysarthria, axial distonia and gait disturbances.
    Defects in NKX2-1 are the cause of choreoathetosis, hypothyroidism, and neonatal respiratory distress (CHNRD) [MIM:610978]. This syndrome include neurological, thyroid, and respiratory problems.
  • 序列相似性Belongs to the NK-2 homeobox family.
    Contains 1 homeobox DNA-binding domain.
  • 翻译后修饰Phosphorylated on serine residues.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AV026640 antibody
    • BCH antibody
    • Benign chorea antibody
    • BHC antibody
    • Homeobox protein NK 2 homolog A antibody
    • Homeobox protein NK-2 homolog A antibody
    • Homeobox protein Nkx 2.1 antibody
    • Homeobox protein Nkx-2.1 antibody
    • Homeobox protein Nkx2.1 antibody
    • NK 2 antibody
    • NK 2 homolog A antibody
    • NK2 antibody
    • NK2 homeobox 1 antibody
    • NK2, drosophila, homolog of, A antibody
    • NK2.1, mouse, homolog of antibody
    • Nkx 2 1 antibody
    • NKX 2.1 antibody
    • NKX 2A antibody
    • NKX2 1 antibody
    • Nkx2-1 antibody
    • NKX2.1 antibody
    • NKX21_HUMAN antibody
    • NKX2A antibody
    • T EBP antibody
    • T/EBP antibody
    • TEBP antibody
    • Thyroid nuclear factor 1 antibody
    • Thyroid nuclear factor antibody
    • Thyroid specific enhancer binding protein antibody
    • Thyroid transcription factor 1 antibody
    • Tin man antibody
    • Tinman antibody
    • TITF 1 antibody
    • TITF1 antibody
    • TTF 1 antibody
    • TTF-1 antibody
    • TTF1 antibody
    see all

Anti-TTF1 antibody [8G7G3/1] 图像

  • ab72876 staining TTF1 in Mouse thyroid tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with 0.1% Tween 20 and blocked with 10% serum for 30 minutes at 24°C. Samples were incubated with primary antibody (1/50 in 10% goat serum in PBS) for 12 hours at 4°C. An Alexa Fluor® 488-conjugated Goat anti-mouse IgG polyclonal (1/500) was used as the secondary antibody.

    See Abreview

  • ab72876 at 1/50 dilution staining TTF1 in human lung by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue.
  • Overlay histogram showing A549 cells stained with ab72876 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab72876, 20µl/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in A549 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-TTF1 antibody [8G7G3/1] (ab72876)参考文献

This product has been referenced in:
  • Nakayama KH  et al. Tissue specificity of decellularized rhesus monkey kidney and lung scaffolds. PLoS One 8:e64134 (2013). IHC-P . Read more (PubMed: 23717553) »
  • Nat R  et al. Pharmacological Modulation of the Hedgehog Pathway Differentially Affects Dorsal/Ventral Patterning in Mouse and Human Embryonic Stem Cell Models of Telencephalic Development. Stem Cells Dev : (2012). Human, Mouse . Read more (PubMed: 22204396) »

See all 3 Publications for this product

Product Wall

The concentration for ab72876 Lot# GR143259-6 is 0.52mg/ml.

The concentration of ab72876, Anti-TTF1 antibody [8G7G3/1] , lot#GR105660-1 is 0.52 mg/ml.

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
Sample Mouse Tissue sections (mouse thyroid)
Specification mouse thyroid
Permeabilization Yes - 0.1% Tween 20
Fixative Paraformaldehyde

Mr. Sanjay Gawade

Verified customer

提交于 Jun 26 2013

Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

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Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.
I would like to reassure you that this antibody is tested and covered by our 6 month guarantee for FlowCytome...

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Thank you for contacting us.

The concentration of lot GR76703-1 is 0.43 mg/ml.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Merci pour ce complément d'informations qui va nous permettre de chercher l'origine du problème rencontré avec le ab40880.

Comme convenu j'ai mis en place l'envoi d'une unité de ab72876, le numéro de commande...

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Thank you for contacting us.

The concentration of the lot you have is 0.43 mg/ml.

We have no western blotting data and the application was added to the datasheet based on a mistaken assumption that the following reference used the...

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Thank you for contacting us. Based on the Uniprot information for Rat TTF1 (, the expected weight based on sequence is around 35 kDa. Real world weight may differ due to factors such as post translational modifi...

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