概述

  • 产品名称Anti-TTC5抗体
    参阅全部 TTC5 一抗
  • 描述
    兔多克隆抗体to TTC5
  • 经测试应用适用于: WB, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    A 14 amino acid peptide from near the carboxy terminus of human TTC5.

  • 阳性对照
    • RAW264.7 cell lysate.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab36855 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa).
IHC-P Use a concentration of 10 µg/ml.
ICC/IF Use a concentration of 5 µg/ml.

靶标

  • 相关性Tetratricopeptide repeat protein 5 (TTC5) is a member of a diverse group of functionally distinct proteins that are characterized by containing one or more tetratricopeptide repeats. Each motif consists of two anti-parallel a-helices such that tandem arrays of TPR motifs generate a right-handed helical structure with an amphipathic channel that may serve to accommodate the complementary region of a target protein. While the exact function of TTC5 remains unclear, it is thought that the TPR motifs serve to mediate protein-protein interactions such as those seen with protein chaperones HSP70 and HSP90 and some proteins involved in cell stress response signaling pathways such as protein phosphatase 5, suggesting that TTC5 may also function via protein-protein interactions mediated by its TPR motifs.
  • 细胞定位Nucleus. Cytoplasm. Note: Phosphorylation at Ser-203 results in nuclear localization, while unphosphorylated protein localizes to the cytoplasm
  • 数据库链接
  • 别名
    • Strap antibody
    • Stress-responsive activator of p300 antibody
    • Tetratricopeptide repeat domain 5 antibody
    • TPR repeat protein 5 antibody

Anti-TTC5 antibody 图像

  • Lane 1 : Anti-TTC5 antibody (ab36855) at 0.5 µg/ml
    Lane 2 : Anti-TTC5 antibody (ab36855) at 1 µg/ml
    Lane 3 : Anti-TTC5 antibody (ab36855) at 2 µg/ml

    Lane 1 : RAW264.7 cell lysate
    Lane 2 : RAW264.7 cell lysate
    Lane 3 : RAW264.7 cell lysate

    Lysates/proteins at 15 µg/ml per lane.

    Secondary
    anti-rabbit IgG HRP at 1/10000 dilution

    Predicted band size : 49 kDa
    Observed band size : 49 kDa
  • ab36855 at 10µg/ml staining TCC5 in mouse brain tissue by IHC

  • ICC/IF image of ab36855 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab36855, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor®594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Immunofluorescence of TTC5 in Mouse Brain cells using ab36855 at 20 ug/ml.

Anti-TTC5 antibody (ab36855)参考文献

This product has been referenced in:
  • Davies L  et al. Regulation of glucocorticoid receptor activity by a stress responsive transcriptional cofactor. Mol Endocrinol 25:58-71 (2011). WB ; Human . Read more (PubMed: 21147850) »

See 1 Publication for this product

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