antigen sequence: GEEDIVDFLR RLVESDPQGL HRIHVDGSSG RLQLWHHDYL LGHLDDEGKS TGQSDRGKGA EGLGTYCGLR KSFLYPPQES EPCPQSPSA, corresponding to amino acids 41-129 of Human TTC31.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
||Use at an assay dependent concentration. Predicted molecular weight: 57 kDa.
Contains 3 TPR repeats.
Information by UniProt
- FLJ12788 antibody
- Tetratricopeptide repeat protein 31 antibody
- TPR repeat protein 31 antibody
Western blot - Anti-TTC31 antibody (ab122745)
Lane 1 :
Lane 2 :
Lane 1 : Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10
Lane 2 : Human Tonsil tissue
Predicted band size : 57 kDa
Immunocytochemistry/ Immunofluorescence - Anti-TTC31 antibody (ab122745)
Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus but not nucleoli and cytoplasm. Recommended concentration of ab122745 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTC31 antibody (ab122745)
ab122745 at 1/200 dilution staining TTC31 in Paraffin-embedded Human vagina tissue by Immunohistochemistry.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"