概述
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产品名称Anti-TTC3抗体
参阅全部 TTC3 一抗 -
描述兔多克隆抗体to TTC3
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宿主Rabbit
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经测试应用适用于: ICC/IF, IHC-Pmore details
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种属反应性与反应: Human
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免疫原
antigen sequence, corresponding to amino acids 19-145 of Human TTC3.
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阳性对照
- Human liver tissue.
性能
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形式Liquid
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存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
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存储溶液pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol -
Concentration information loading... -
纯度Immunogen affinity purified
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克隆多克隆
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同种型IgG
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研究领域
相关产品
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Compatible Secondaries
应用
Our Abpromise guarantee covers the use of ab121783 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | Use a concentration of 1 - 4 µg/ml. Fixation/Permeabilization: PFA/Triton X-100 | |
| IHC-P | 1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能E3 ubiquitin-protein ligase that mediates the ubiquitination and subsequent degradation of phosphorylated Akt (AKT1, AKT2 and AKT3) in the nucleus. Acts as a terminal regulator of Akt signaling after activation; its phosphorylation by Akt, which is a prerequisite for ubiquitin ligase activity, suggests the existence of a regulation mechanism required to control Akt levels after activation. Catalyzes the formation of 'Lys-48'-polyubiquitin chains. May play a role in neuronal differentiation inhibition via its interaction with CIT.
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组织特异性Found in all tissues examined.
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通路Protein modification; protein ubiquitination.
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序列相似性Contains 1 RING-type zinc finger.
Contains 4 TPR repeats. -
翻译后修饰Phosphorylation on Ser-378 by Akt is required for ubiquitin ligase activity.
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细胞定位Nucleus.
- Information by UniProt
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数据库链接
- Entrez Gene: 7267 Human
- Omim: 602259 Human
- SwissProt: P53804 Human
- Unigene: 368214 Human
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别名
- DCRR1 antibody
- E3 ubiquitin protein ligase TTC3 antibody
- E3 ubiquitin-protein ligase TTC3 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTC3 antibody (ab121783)ab121783, staining TTC3 in paraffin-embedded Human Liver tissue, by Immunohistochemistry. -
Immunocytochemistry/ Immunofluorescence - Anti-TTC3 antibody (ab121783)ab121783, at 4 µg/ml, staining TTC3 (green) in PFA-fixed, Triton-permeabilized Human A-431 cells, by Immunofluorescence.
文献
ab121783 has not yet been referenced specifically in any publications.
客户评价及客户问答
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"