概述

  • 产品名称Anti-TTC11抗体
    参阅全部 TTC11 一抗
  • 描述
    兔多克隆抗体to TTC11
  • 经测试应用适用于: ICC/IF, WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Cow
  • 免疫原

    Recombinant fragment containing a sequence corresponding to a region within amino acids 1-122 of Human TTC11 (NP_057152).

  • 阳性对照
    • Raji whole cell lysate; NCIN87 xenograft; 293T, HeLa and Molt-4 lysates

性能

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应用

Our Abpromise guarantee covers the use of ab96764 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 µg/ml.
WB 1/500 - 1/3000. Predicted molecular weight: 17 kDa.
IHC-P 1/100 - 1/500.

靶标

  • 功能Promotes the fragmentation of the mitochondrial network and its perinuclear clustering. Can induce cytochrome c release from the mitochondrion to the cytosol, ultimately leading to apoptosis. Also mediates peroxisomal fission.
  • 序列相似性Belongs to the FIS1 family.
    Contains 1 TPR repeat.
  • 结构域The C-terminus is required for mitochondrial or peroxisomal localization, while the N-terminus is necessary for mitochondrial or peroxisomal fission, localization and regulation of the interaction with DNM1L.
  • 翻译后修饰Ubiquitinated by MARCH5.
  • 细胞定位Mitochondrion outer membrane. Peroxisome membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 2010003O14Rik antibody
    • CGI 135 antibody
    • CGI135 antibody
    • FIS 1 antibody
    • FIS1 antibody
    • Fis1 homolog antibody
    • FIS1, S. cerevisiae, homolog of antibody
    • FIS1_HUMAN antibody
    • Fission 1 (mitochondrial outer membrane) homolog (S. cerevisiae) antibody
    • Fission 1 (mitochondrial outer membrane) homolog (yeast) antibody
    • Fission 1 (mitochondrial outer membrane) homolog antibody
    • Fission 1 homolog antibody
    • H NH0132A01.6 antibody
    • hFis 1 antibody
    • hFis1 antibody
    • Mitochondrial fission 1 protein antibody
    • Tetratricopeptide repeat domain 11 antibody
    • Tetratricopeptide repeat protein 11 antibody
    • TPR repeat protein 11 antibody
    • TTC 11 antibody
    see all

Anti-TTC11 antibody 图像

  • Anti-TTC11 antibody (ab96764) at 1/10000 dilution + Raji whole cell lysate at 30 µg

    Predicted band size : 17 kDa
  • Immunohistochemical analysis of TTC11 in paraffin-embedded NCIN87 xenograft, using ab96764 antibody at 1/100 dilution.
  • ICC/IF image of ab96764 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab96764, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-TTC11 antibody (ab96764)参考文献

This product has been referenced in:
  • Kitaoka Y  et al. Combined effects of resistance training and calorie restriction on mitochondrial fusion and fission proteins in rat skeletal muscle. J Appl Physiol (1985) 121:806-810 (2016). Read more (PubMed: 27539498) »
  • Cho B  et al. CDK5-dependent inhibitory phosphorylation of Drp1 during neuronal maturation. Exp Mol Med 46:e105 (2014). WB . Read more (PubMed: 25012575) »

See all 2 Publications for this product

Product Wall

Application Western blot
Loading amount 40 µg
Gel Running Conditions Reduced Denaturing (10% SDS PAGE)
Sample Mouse Tissue lysate - whole (Liver)
Specification Liver
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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提交于 Jul 15 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"